Eppendorf R Series 48R CO2 Incubator - Manual (Page 56)

56
CO48R-0050 Š Galaxy R Series 48 R Personal Size CO
2
Incubator User’s Guide
8.6 O
2
Control (0.1-19%)
This oxygen control option uses the controlled addition of nitrogen to reduce the
oxygen level below ambient. If you have the 1-19% oxygen control option, go back to
Section 8.5; or if you have the 1-95% oxygen control option, skip ahead to Section 8.7.
8.6.1 Setting Up the N
2
Tank
Before you set up your oxygen control, be sure you have the proper equipment
for your nitrogen supply:
• 2 cylinders of nitrogen, regulation size W
• 1 two-stage pressure regulator
• 1 Inline pressure regulator
• 6mm PVC tubing
• tubing clips
Set up the nitrogen tanks as follows:
1. Inspect them to be sure there are no leaks or other damage.
2. Check that the two-stage pressure regulator valve and the inline pressure
regulator valve are closed by trying to turn their knobs in a counter-
clockwise direction; the knobs should be at the stop point.
3. Place the inline pressure regulator close to the incubator.
4. Securely attach the two-stage pressure regulator to the nitrogen tank’s outlet
and to the inline pressure regulator’s inlet with tubing, securing both ends
with clips.
5. Connect the inline pressure regulator’s outlet to the incubator’s N
2
inlet on
the right side of the equipment tray at the rear of the incubator (see Figure
7); secure both ends with clips.
6. Proceed as indicated in Section 8.6.2.
NOTE:
To add an automatic gas cylinder changeover instrument: with the
incubator turned off, connect its inlet to the two-stage pressure
regulator’s outlet, and the changeover instrument’s outlet to the inline
pressure regulator’s inlet with tubing, securing the ends with tubing clips.
8.6.2 Setting Up O
2
Control
Oxygen control is tailored such that both the O
2
and the CO
2
levels are achieved
at approximately the same time, via control of the N
2
valve’s duty cycle (but
only within the range of 0.1-19% O
2
).