Eppendorf R Series 48R CO2 Incubator - Manual (Page 59)

59
New Brunswick Scientific User’s Guide
8.6.7 Precautions
Under normal humidity conditions (95-99% RH), the sensor is unaffected. If
for any reason (such as a large spill inside the chamber or the incubator being
switched off while fully humidified) liquid condenses around the sensor, the
result may be restriction of gas flow and a low sensor signal. Should such
condensation appear on the chamber walls, normal operation can be easily
restored by removing the humidity tray, drying the chamber completely, and
then running the incubator at 37°C for one hour. This will dry out the sensor.
After the hour has elapsed, the humidity tray can be reinstalled and the
incubator humidified again.
8.7 O
2
Control (1-95%)
This oxygen control option uses the controlled addition of oxygen to increase the level
above ambient and the controlled addition of nitrogen to reduce the oxygen level below
ambient. If you have the 1-19% oxygen control option, go back to Section 8.5; or for
the 0.1-19% oxygen control option, go back to Section 8.6.
If your incubator is operating in the 3-95% range of O
2
control, only the N
2
valve will
operate for setpoints below 19%; both the N2 and O2 valves will operate for setpoints
between 19 and 80%; and for setpoints above 80%, only the O2 valve will operate.
NOTE:
If you plan to maintain O
2
levels of 80-95%, be sure to read Section 8.7.4.
WARNING!
High oxygen levels inside the chamber require additional safety
precautions:
ΠSetting the oxygen to levels >24% can greatly increase the chance of fire
and explosion. Operators planning to set oxygen at 24-95% should
therefore take all appropriate precautions to minimize the risk. NBS does
not recommend using external (heat-generating) equipment inside the
chamber when oxygen conditions will be >24%.
ΠBefore opening the incubator door, make sure no flames (Bunsen
burner, etc.) are in the vicinty.
ΠAvoid the presence of other combustible gases (hydrogen, methane,
etc.)
ΠEnsure that all solenoid valves and pressure regulators & gauges
controlling the gas supply are grease-free for use with oxygen.