Luminex MAGPIX Hardware and Installation Manual - Manual (Page 94)

• MAGPIX calibrators
• MAGPIX performance verifiers
• assay standards
• assay controls
• error messages
Review the log of calibration reports routinely to detect trends.
Use MAGPIX performance verifiers to check the success of the instrument calibration and to
troubleshoot. If there is a problem with kit results, MAGPIX performance verifiers can help
determine if the problem is related to the instrument. If calibration and verification are
successful, contact the kit manufacturer.
The following table identifies the three possible categories of microsphere dot plots: normal,
irregular due to photo bleaching of the microspheres, and irregular due to MAGPIX being out
of calibration.
TABLE 2. Types of Dot Plots
Appearance Description Possible Cause Corrective
Action
Normal Bead Grouping
MagPlex verifiers
form a tight bead
population within the
boundaries of the
gray region.
Irregular Bead Grouping: Photo bleached Microspheres vs. Out-of-Calibration Instrument
MagPlex verifiers are
outside of their region
(above or to the
right). Instrument is out of
calibration. Recalibrate and
verify.
MagPlex verifiers are
outside of their region
(below or to the left). MagPlex calibrators
are Photo bleached. Recalibrate with
new MagPlex
calibrators and
verify.
Irregular Bead Grouping due to Out-of-Calibration Instrument
Troubleshooting Procedures
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