Luminex MAGPIX Hardware and Installation Manual - Manual (Page 93)

Possible Cause Corrective Action
There are too few or too many xMAP
microspheres in the well. Ensure that there are 2000 to 5000 beads per
bead set per well.
The xMAP microspheres have expired. Substitute an unexpired bottle of xMAP
microspheres.
The xMAP microspheres are photo bleached. Substitute xMAP microspheres that are not photo
bleached.
Possible Sample Probe Causes:
The sample probe height is incorrect. Perform an automatic sample probe height
adjustment.
The sample probe is clogged. Removing Clogs on page 62.
Other Possible Causes:
The Drive Fluid or waste line is not fully
connected. Disconnect and reconnect the lines.
Air is present in the instrument. Verify the sample probe height. Use the software
to run a prime command three times, an alcohol
flush command twice, then a wash command
three times with distilled water.
Make sure the Drive Fluid coil is not pinched.
The acquisition volume is set too high. Set the acquisition volume to at least 25 µL less
than the actual volume in your wells. This enables
the probe to acquire sample more efficiently with
less chance of incorporating air in the sample.
The sample is too concentrated. Dilute concentrated biological fluids, such as
serum or plasma, at least 1:5.
Carryover Problems
Carryover from calibration can interfere with verification; likewise, carryover from an assay
can interfere with correct reading of the following assay. Take the following steps to eliminate
carryover:
• Begin by performing four rinse cycles.
• If that fails, run the Clean command twice using a 0.1N sodium hydroxide solution.
• If running the Clean command twice fails, run the Enhanced Start Up Routine.
Bead Detail Irregularities
Use these tools to assist in diagnosing instrument vs. kit-related problems:
MAGPIX ®
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