Luminex MAGPIX Hardware and Installation Manual - Manual (Page 90)

Calibration Failure
Calibration problems can result from a variety of causes, many of them easily correctable
human errors. The following error codes are associated with calibration failure:
• Error code 59: Normalizer has a zero value. Re-calibrate.
• Error code 2054: Insufficient bead count, beads may be out of focus. Self-test may help by
re-homing camera.
• Error code 8226: Bubbles sense error.
• Error code 2091: General hardware error was encountered. Self-test to confirm.
Possible Cause Corrective Action
Possible Calibration Microsphere Causes:
The calibration microspheres are not fully
resuspended. Vortex the calibration vials to resuspend the
microspheres.
The wrong lot number or target values were
entered in the software. Correct the lot number and target values in the
software.
The calibration microspheres are in the wrong
well. Change the well setting in the software.
There are not enough calibration microspheres in
the well. Add at least six drops of calibration microspheres
to the well. For accurate drop volume, hold the
vial upside down at a 90 degree angle to the
microtiter plate while dispensing them.
You are using the incorrect calibration
microspheres. Use the xMAP MAGPIX Calibration Kit.
The calibration microsphere lot is expired. Substitute an unexpired bottle of calibrator
microspheres.
The calibration microspheres are photobleached. Use a different bottle of calibrator microspheres,
one which has been protected from light during
storage.
Possible Sample Probe Causes:
The sample probe height is incorrect. Perform an automatic sample probe height
adjustment.
The sample probe is clogged. Clogs on page 75.
The probe fitting is loose. Tighten the probe fitting.
Other Possible Causes:
There is a partial clog in the instrument. Clogs on page 75.
Troubleshooting Procedures
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