Waters Oligonucleotide Separation Technology ACQUITY UPLC C18 Columns - Manual (Page 5)

[ Care and Use ManUal ]
5
Number 430001486) is required for 425 µL mixer installation onto
a standard ACQUITY UPLC
® System.
Note: The 425 µL mixer introduces an additional delay volume to
gradient separations. For ultra-fast OST analyses, the smaller
50 µL mixer should be used with the described premixed mobile
phase technique.
h. flow rate
The recommended flow rate for oligonucleotide separations performed
on a 2.1 x 50 mm ACQUITY UPLC
® OST C
18
column is 0.2 mL/minute.
When faster flow rates are desired for separations, use of the 425 µL
mixer with installed Outlet Tubing Assembly is recommended.
III. Column CleanInG, reGeneratInG and StoraGe
a. Cleaning and regeneration
Changes in peak shape, peak splitting, shoulders on the peak, shifts
in retention, change in resolution or increasing backpressure may
indicate contamination of the column. Flushing with approximately
20 column volumes of 0.2 μm membrane filtered, neat organic
solvent (e.g., acetonitrile with the TEAA method of methanol with the
TEA/HFIP protocol) is usually sufficient to remove the contaminant.
If the neat organic solvent flushing procedure does not solve the
problem, purge the column with 20 column volumes of OST mobile
phase A followed by 20 column volumes of either 7M guanidine
hydrochloride or 7M urea. Be sure to flush column with an additional
20 column volumes of 0.2 μm membrane filtered, LC Grade Water
prior to reuse of OST mobile phases. If the column performance is
poor after regenerating and cleaning, call your local Waters office for
additional support.
b. Storage
For periods longer than four days at room temperature, store the
column in 100% acetonitrile. Immediately after use at elevated
temperature and/or pH, store column in 100% acetonitrile for
the best column lifetime. Do not store column in highly aqueous
(<20% organic) mobile phase, as this may promote bacterial growth.
Completely seal column to avoid evaporation and drying out of the
packed bed.
IV. eCord ™ IntellIGent ChIp teChnoloGy
a. Introduction
The eCord
™ intelligent chip is a new technology that will provide the
history of a column’s performance throughout its lifetime. The eCord ™
is permanently attached to the column to assure that the column’s
performance history is maintained in the event that the column is
moved from one instrument to another.
At the time of manufacture, tracking and quality control information
will be downloaded to the eCord™. Storing this information on the
chip will eliminate the need for a paper Certificate of Analysis.
Once the user installs the column, the software will automatically
download key parameters into a column history file stored on the
chip. The eCord™ provides a solution to easily track the history of
column usage.
Waters eCord ™ - intelligent chip
eCord ™ inserted into side of
column heater