Waters Oligonucleotide Separation Technology ACQUITY UPLC C18 Columns - Manual

[ Care and Use ManUal ]
Thank you for choosing an ACQUITY UPLC ® OST C
18
column designed
specifically for use on Waters ACQUITY UPLC ® System. The separa-
tion of detritylated oligonucleotides on Waters second generation of
hybrid-silica BEH Technology
™ particles are based on the well estab-
lished method on ion-pair, reversed-phase chromatography. ACQUITY
UPLC
® OST C
18
columns are available in several configurations to
address different application needs. Your ACQUITY UPLC ® OST C
18
packing material is manufactured in a cGMP, ISO 9002 certified plant
using ultra pure reagents. Each batch of ACQUITY UPLC
® OST C
18
material is chromatographically tested with acidic, basic and neutral
analytes and the results are held to narrow specification ranges to
assure excellent, reproducible performance. In addition, every column
is individually tested and the associated Performance Test Chromato-
gram and Certificate of Acceptance information is available through
the attached eCord
™ intelligent chip technology.
Note: Optimum performance of ACQUITY UPLC ® OST C
18
columns is
best assured using an appropriately configured Waters ACQUITY UPLC ®
System (e.g., See Section II, g). Consequently, use of ACQUITY UPLC ®
OST C
18
columns on conventional HPLC systems is not recommended.
Contents
I. GettInG Started
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord™ Installation
e. Initial Column Efficiency Determination
II. Column uSe
a. Sample Preparation
b. Recommended Mobile Phases
c. Recommended Injector Wash Solvents
d. pH Range
e. Pressure
f. Temperature
g. Mixer Options
h. Flow Rate
III. Column CleanInG, reGeneratInG and StoraGe
a. Cleaning and Regeneration
b. Storage
IV. eCord™ IntellIGent ChIp teChnoloGy
a. Introduction
b. Installation
V. addItIonal InformatIon
a. Band Spreading Minimization
ACQUIt Y UPLC ® ost C
18
*ost: oLIgonUCLeotIde sePArAtIon teChnoLogY