Care and use manual – Waters NanoEase Trapping Columns User Manual

Page 3

[ Care and Use ManUal ]

NanoEase Trapping Columns

c) Grip the outside of black fitting (Figure 1a and 1b) and care-

fully screw it into the valve port to seal the trap into the
valve, while being careful not to rotate the column-blanking
plug.

d) Check to see that the black fitting is tightly sealed into the

valve, but do not overtighten it.

e) Secure the tubing to form the “sample loop” (fused silica, or

60 mm i.d. tan PEEK) into the top of the trap column with an
appropriate fitting (see Figure 3).

5. For Nano LC applications using 75 μm analytical columns, dead

volume can be minimized when using a multi-port valve by
placing the trap column at the closest end to the inlet of the
analytical column. Figures 4 and 5 show the plumbing diagram of
such a configuration for the wash and elute cycles using a 10-port
switching valve.

6. Connect up the HPLC System and start flow through the

NanoEase Trap Column. Check for leaks and tighten the fittings
if necessary.

iii. GEnEral GuidElinEs for column usE

Typical Separation Flow Rates: 1-40 µL/min

Typical Loading Flow Rate: 15 µL/min for 4 minutes (with Waters CapL

and

Stream Select Module)

Loading Capacity: 2-5 µg maximum for the RP; 4-8 µg maximum for the SCX
(will vary depending on sample)

Storage Recommendations: Long term storage in acetronitrile for the RP;
Storage in aqueous solvent is adequate for SCX (e.g. HPLC grade water with
acetronitrile).

Typical Column Lifetime: Varies depending on sample and care of column,
but excess of 100 injections is possible.

Figure 4. Loading and wash step

Figure 5. Elution step

NanoEase

trap column

25 mm i.d. fused silica tubing

to nanoLC
column

7 6 5

waste

10-Port

Valve

auxiliary

pump

A/S

valve

nanoLC gradient

10-32 fitting with

fused silica sleeve