Waters NanoEase Trapping Columns - Manual

[ Care and Use ManUal ]
NanoEase Trapping Columns 1
i. introduction:
Waters NanoEase Trapping Columns are robust devices that can effectively
separate complex samples and remove buffers and high concentrations of
salts. For the latest on Life Science Chemistries, go to:
www.waters.com/lifesciences.
ii. nanoEasE trap column prEparation
1. It is recommended that the trap column be washed and conditioned
with a suitable solvent before use. This can be accomplished using
a suitable syringe to flush solvent through the column. If a syringe
is used for this purpose, it is recommended that a syringe with an
inert teflon plunger and glass body be used to avoid contami-
nation. Syringes with rubber plungers or plastic bodies should
not be used. Alternatively, the trap column can be placed on the
HPLC system and washed with suitable solvent at a flow rate of
10-40 µL/min for several minutes prior to use.
2. If using a 10-port valve, use UpChurch ® long nuts (UpChurch
part # F-285X) in each adjacent port of the 10-port valve, due to
the clearance width of the trap column.
3. The trap column works with the black 10-32 outer fitting with
white label (Figures 1a and 1b), which secures the stainless steel
tube and the tan PEEK
® (column insert part) piece (Figure 2) to the
switching valve.
contEnts
i. introduction
ii. nanoEasE trap column prEparation
iii. GEnEral GuidElinEs for column usE
iv. ordErinG information
nanoEasE trappinG columns