Waters MassPREP Phosphopeptide Standard - Manual (Page 2)

[ Care and Use ManUal ]
MassPREP Phosphopeptide Standard – Enolase 2
IV. example spectra
The following MALDI TOF MS spectrum of MassPREP Phosphopeptide Standard
– Enolase (2 pmol/sample well) mixed with MassPREP MALDI Matrix - DHB
(20 mg/mL) was processed by baseline subtraction. Data in positive reflectron
mode was obtained on a Waters MALDI micro MX
™ (sum of 200 laser shots).
The following LC-ESI-MS in positive mode (top) and LC-UV at 20 nm (bottom)
spectra of the MassPREP Phosphopeptide Standard – Enolase was obtained
using a Waters Alliance
® 2795 coupled to a Waters ZQ ™ 4000 mass spec-
trometer. A 20 pmol/μL solution in 0.02% trifluoroacetic acid in water was
prepared and 30 μL was injected on a Waters BioSuite C8 3 μm, PA – A,
2. x 50 mm column.
T43
T18
T43
T19
m/z
500 600 700 800 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900
%
0
100
1368.72
813.51
863.52
1448.65
Ti me
12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00
A
U
0.0
2.0e-2
4.0e-2
6.0e-2
8.0e-2
210
Range: 9.871e-2
Area
17.34
14867
15.69
9416
22.38
11337
24.61
10585
T18 1P
T19
T43
Ti me
10.00 12.50 15.00 17.50 20.00 22.50 25.00 27.50 30.00
%
0
100
T43
T43 2P
T18 1P
T43
T19