Waters HSPgel Columns - Manual (Page 4)

[ Care and Use ManUal ]
HSPgel Columns for High Speed GPC Analysis 4
c. Solvents
To maintain maximum column performance, use high-quality chromatography
grade solvents. It is recommended to degas all solvents thoroughly before use
to prevent bubble formation in the pump and detector. The use of an on-line
degassing unit is also recommended. This will help to maintain a stable RI
detector baseline
.
Table 3. Availible Solvents for HSPgel Columns
* HR columns may be switched from THF to one of the listed solvents one time only -
at 0.10 mL/minute.
** RT columns may be switched back and forth to the listed solvents at the flow rates shown
in Table 4.
*** HT columns are shipped in either THF or ODCB. For work in TCB, the ODCB columns may
be directly switched over at 0.15 mL/min at >100 °C.
**** AQ columns may be used with up to 50% organic loading (methanol or acetonitrile).
Table 4. Recommended Flow Rates for Solvent Switchover
* (cp) - centiopise
Application Columns
Recommended
Flow Rate**
Maximum
Flow Rate
Maximum
Pressure
Drop**
Ultra-Hgh
Resolution
GPC
HR 1.0 0.25- 0.60 0.70 380
HR 2.0 0.25- 0.60 0.70 345
HR 2.5 0.25- 0.60 0.70 275
HR 3.0 0.25- 0.60 0.70 240
HR 4.0 0.25- 0.60 0.70 240
HR MB-L 0.25- 0.60 0.70 240
HR MB-M 0.25- 0.60 0.70 140
HR MB-H 0.25- 0.60 0.70 70
Room
Temperature
GPC
RT 1.0 0.30- 0.70 0.80 480
RT 2.0 0.30- 0.70 0.80 480
RT 2.5 0.30- 0.70 0.80 415
RT 3.0 0.30- 0.70 0.80 415
RT 4.0 0.30- 0.70 0.80 275
RT 5.0 0.30- 0.70 0.80 275
RT 6.0 0.30- 0.70 0.80 210
RT 7.0 0.30- 0.70 0.80 210
RT MB-L 0.30- 0.70 0.80 275
RTMB-L/M 0.30- 0.70 0.80 275
RTMB-M 0.30- 0.70 0.80 275
RTMB-H 0.30- 0.70 0.80 275
High
Temperatire
GPC
HT 1.0 0.30- 0.70 0.80 415
HT 2.0 0.30- 0.70 0.80 415
HT 2.5 0.30- 0.70 0.80 415
HT 3.0 0.30- 0.70 0.80 415
HT 4.0 0.30- 0.70 0.80 415
HT 5.0 0.30- 0.70 0.80 415
HT 6.0 0.30- 0.70 0.80 415
HT 7.0 0.30- 0.70 0.80 415
HT MB-L 0.30- 0.70 0.80 415
HTMB-L/M 0.30- 0.70 0.80 415
HTMB-M 0.30- 0.70 0.80 415
HTMB-H 0.30- 0.70 0.80 415
Aqueous AQ 2.5 0.30- 0.60 0.70 415
AQ 3.0 0.30- 0.60 0.70 415
AQ 4.0 0.30- 0.60 0.70 245
AQ 5.0 0.30- 0.60 0.70 210
AQ 6.0 0.30- 0.60 0.70 140
AQ MB-H 0.30- 0.60 0.70 140
Table 2. Flow-Rate Specifications for HSPgel Columns
* Flow rates in mL/min.
** Pressure drops in PSI. Increasing column temperature can dramatically decrease.
Column
Type
Shipment Solvent Chargeable Solvent
HR* Tetrahydrofuran Toluene, xylene, chloroform dichlromethane,
(methylene chloride), dichloroethane
RT** Tetrahydrofuran Toluene, xylene, chloroform, dichlromethane,
(methylene chloride), dichloroethane,
dimethylfomamide, dimethylacetamide,
n-methyl pyrrolidine hexafluoroisopropanol,
(HFIP), DMSO
HT*** THF or ODCB Same as RT, and also: ODCB, TCB,
o-chlorophenol, 1-chloronapthalene
AQ**** Water Water with salts/buffers up to 0.80M,
pH 2 -12, 50% methanol or acetonitrile
Solvent
RT Flow Rate
(mL/min)
HT Flow Rate
(mL/min)
Viscosity (cp) *
(at temperature
shown)
Toluene 0.30 0.30 0.59 (20 °C)
Dichloromethane 0.30 0.30 0.43 (20 °C)
Chloroform 0.30 0.30 0.56 (20 °C)
DMF/DMAC 0.20 0.25 0.7 - 0.8 (25 °C)
DMSO 0.10 0.15 2.0 (25 °C)
NMP 0.10 0.15 1.65 (25 °C)
ODCB/TCB 0.10 0.15 1.3 - 1.5 (25 °C)
HFIP 0.05 0.05 1.32 (25 °C)
1-chloronapthalene – 0.05 2.94 (20 °C)
THF – – 0.55 (20 °C)