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Waters HSPgel Columns User Manual

Care and use manual, Hspgel columns for high-speed gpc analysis

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[ Care and Use ManUal ]

HSPgel Columns for High Speed GPC Analysis

1

Contents

I. IntroduCtIon

II. HsPgel Column FamIlIes

III. ConneCtIng tHe Column to tHe gPC system

a. Column Connectors
b. Connecting an HSPgel Column
c. Solvents

IV. Column temPerature ranges

I. IntroduCtIon

Thank you for choosing a Waters HSPgel

column. Every column

is individually tested and Certificates of Batch Analysis and a
Performance Chromatogram are provided with each column. It is not
recommended to use a guard column with the HSPgel columns, as it
will add significant volume and possible additional bandspreading
to the system. It is recommended, however, that a ~0.5 µm in-line
filter (pre-column) be installed to trap any particulates that may
potentially plug the column frit. The GPC analysis solvent must
be filtered prior to use through an appropriate ~0.5 µm filter. The
sample solutions should also be filtered prior to injection, unless the
sample molecular weight is very high and there may be a chance of
shear degradation.

HsPgel Columns For HIgH-sPeed gPC analysIs