Waters Pico-Tag Columns for Free Amino Acids - Manual (Page 4)

[ Care and Use ManUal ]
The Pico•Tag Column for Free Amino Acids 4
Test Sample: Acenaphthene (0.05% in Acetonitrile)
Solvent: Pico•Tag Eluent B (P/N 88112)*
Flow Rate: 1.0 ml/min
Chart Speed: 10 cm/min
Injection Volume: 8 µl
Detection: 254 nm @ 0.2 AUFS
*or equivalent made by mixing 600 ml of HPLC grade acetonitrile with 400
ml Milli-Q° water.
b. Troubleshooting
Table 1: Typical Column Problems and Solutions
V. column storage
Leaving the column unused for less than 72 hours does not generally require
storage procedures. Follow the procedures below to store column for periods
of more than 72 hours.
• DO NOT store the column in water alone; this practice will promote
microbial contamination. Store the column in Pico•Tag Eluent 2.
• DO NOT allow buffers or other potentially harmful materials to remain
in the system when not being used. Flush and replace with Pico•Tag
Eluent 2 or 10% organic in water mixture.
• Return the column to its box with the end plugs firmly in place for stor-
age. Allowing steel columns to dry out can result in poor chromatogra-
phy performance.
VI. warranty/serVIce InformatIon
a. Service and Applications Assistance
Waters’ staff of experienced service specialists provide maintenance assis-
tance on both preventative and/or corrective levels. For complete information
and assistance, please call Waters Service Department at 1-800-252-HPLC.
For solutions to particular applications questions Waters team of technical
support personnel are available to help you with specialized support. They may
be contacted at 1-800-252-HPLC in Milford.
b. Warranty
Warrants its high performance liquid chromatography columns in accordance
with the following terms and conditions:
Waters will repack or replace (at our discretion) without cost any steel col-
umn that fails to perform satisfactorily if notified within 90 days from your
receipt. Any column that is returned must have a Return Authorization Number
granted by the Waters Customer Ser-vice Department. Approval is subject to
the following exclusions:
• Physical damage to the column because of misuse or abuse.
• Chemical damage to the packing material because of use with incompat-
ible solvents or buffers, or at an incorrect pH.
• Physical damage to the packing material because of operation at incor-
rect temperatures or pressures.
• Particulate buildup or precipitation in the column or end fittings causing
high internal pressure which has occurred because of improper solvent
or sample filtration practices.
VII. orderIng InformatIon
Problem Cause Solution
Excess pressure
buildup
• Filters plugged
with Particulates
• Sample precipi-
tates on column
(sample not
soluble in mobile
phase)
• Clean in an ultrasonic bath
or replace.
• Always alter mobile phases
and samples.
• Slowly purge with a strong
mobile phase that is both
appropriate to dissolve the
contaminate and compat-
ible with the column.
Loss of resolution,
broad peaks, low
plate counts
• Filters partially
plugged
• Contaminated
colum, insufficent
equilibration
• Column collapse
and void formation
• Replace or clean inlet
and outlet filters in an
ultrasonic bath.
Item Part Number
Phenylisothiocyanate (PITC) 88120
Triethylamine (TEA) 88121
Physiological Standard A/N 10948
Physiological Standard B 10949
Pico•Tag Eluent 1 10960
Pico•Tag Eluent 2 10965
Sample Clarification Kit 26865
In-Line Precolumn Filter Kit 84560
Pico•Tag Diluent 88119