Waters Pico-Tag Columns for Free Amino Acids - Manual

[ Care and Use ManUal ]
The Pico•Tag Column for Free Amino Acids 1
I. IntroductIon
Waters Pico•Tag ® Column for Free Amino Acids uses the exclusive bonding
and pack-ing processes developed by Waters for the original Pico•Tag column.
This has resulted in a durable, high efficiency, 3.9 mm x 30 cm column ideally
suited for the reversed-phase separation of phenylthiocarbamyl amino acids.
Our stringent quality control procedures assure that this column, when used
in conjunction with Waters Pico•Tag reagents, eluents, and HPLC system,
will provide the speed, resolution, and sensitivity needed for amino acid
analysis.
Use these procedures to ensure that you obtain quality results from your
Pico•Tag column and take full advantage of the features it offers.
WARNING: The ampules of phenylisothiocyanate which are contained in the
Pico•Tag chemical package react on contact with strong acids, emitting
highly toxic cyanide fumes and/or oxides of sulphur. Care must be taken to
assure that these ampules and contents are disposed of properly and that
they do not come into contact with acids.
contents
I. IntroductIon
II. InstallatIon
a. Solvent Requirements
b. Sample and Solvent Preparation
c. Column and Cartridge Installation
d. Equilibration
III. operatIng tIps
IV. column effIcIency
a. Column Testing
b. Troubleshooting
V. column storage
VI. warranty/serVIce InformatIon
VII. orderIng InformatIon
Pico•Tag column for free amino acids