Waters Pico-Tag Amino Acid Analysis Column - Manual (Page 4)

[ Care and Use ManUal ]
Pico•Tag Amino Acid Analysis Column 4
IV. column eFFIcIency
Waters measures column efficiency by using the 5-sigma method. Plate count,
as an expression of efficiency, is determined by measuring the peak width at
4.4% of the peak height. Unlike half-peak height and tangent methods used by
other manufacturers, this stringent method takes into con-sideration naturally
occurring peak asymmetry.
Figure 3. Column Efficiency
V. test condItIons
Columns are thoroughly tested in our quality control laboratories for adher-
ence to our specifica-tions. Since slight variations in your results will occur
depending on the equipment used, test sam-ple makeup and equipment
settings and condition, perform the test sample run given here for your new
column and record the results (retention time and the settings used) before
attempting the first analysis. Use these results for comparison throughout the
life of your column.
NOTE: BE SURE TO RECORD RESULTS AND INSTRUMENT SETTINGS (AND
CONFIGURATIONS) TO ALLOW EXACT REPRODUCTION AND COMPARISON IN
THE FUTURE.
Test Sample: Acenaphthene (0.05% in acetonitrile)
Solvent: Pico•Tag Eluent B
Flow Rate: 1.0 ml/min
Chart Speed: 10 cm/min
Injection Volume: 15 µl
Detection: 254 nm @ 0.5 AUFS
VI. recommended sPAre PArts (steel columns)
* Included as components of 3.9 mm Filter Replacement Kit
VII. WArrAnty
Waters warrants its quality manufactured columns in accordance with the
following terms and conditions. Waters will repack or replace the column (at
our discretion) at no cost if it fails to perform satisfactorily and Waters is noti-
fied within 90 days of receipt. Columns being returned must have prior Return
Authorization granted by Waters Customer Service Department. Approval is
subject to the following exclusions:
• Physical damage to the column due to misuse or abuse.
• Chemical damage to the packing material due to operating with incom-
patible solvents, buffers, or pH.
• Physical damage to the packing material due to operation at incorrect
temperatures or pressures.
• High internal pressure in the column due to improper solvent or sample
filtra-tion practices causing particulate buildup or precipitation in the
column or end fittings.
Item P/N Quantity
Pico•Tag Reagent Kit 88123 1
Phenylisothiocyanate (PITC) 88120 1
Triethylamine (TEA) 88121 1
Protein Hydrolyzate Standards 88122 1
3.9 mm Filter Replacement Kit 84095 1
Filter Insert Assembly (only)* 84056 2
Retainers (only)* 84057 2