Waters Pico-Tag Amino Acid Analysis Column - Manual

[ Care and Use ManUal ]
Pico•Tag Amino Acid Analysis Column 1
I. IntroductIon
Waters exclusive bonding and packing processes, developed for the Pico•Tag ®
Column (3.9 mm x 15 cm), P/N 88131, produce a durable, high efficiency
stationary phase ideally suited for the reverse-phase separation of phenyl-
thiocarbamyl amino acids. Our stringent quality control procedures result
in a column which, when used in conjunction with Waters Pico•Tag reagents,
eluents, and HPLC system is guaranteed to provide the speed, resolution, and
sensitivity needed for amino acid analysis.
This information provides the means to ensure that you derive the full benefit
of our research, manufacturing, and quality control. For additional information
on the complete method, refer to Section 4.4 of the Amino Acid Analysis
Manual, P/N 07124, or the Pico•Tag Amino Acid Analysis System Operator’s
Manual, P/N 88140.
WARNING: THE AMPULES OF PHENYLISOTHIOCYANATE WHICH ARE
CONTAINED IN THE PICO•TAG CHEMICAL PACKAGE REACT ON CONTACT WITH
STRONG ACIDS, EMITTING HIGHLY TOXIC CYANIDE FUMES AND/OR OXIDES
OF SULPHUR. CARE MUST BE TAKEN TO ASSURE THAT THESE AMPULES AND
CONTENTS ARE DISPOSED OF PROPERLY AND THAT THEY DO NOT COME INTO
CONTACT WITH ACIDS.
Figure 1. Pico•Tag Method Amino Acid Analysis 250 pmol of
Pierce H Standard
contents
I. IntroductIon
II. PrePArAtIon For oPerAtIon
a. Column Installation
b. Solvent Requirements
c. Equilibration
III. cAre And use
a. Sample and Eluent Preparation and Filtration
b. Precautions
c. Storage Considerations
d. Troubleshooting
e. Service and Applications Information
IV. column eFFIcIency
V. test condItIons
VI. recommended sPAre PArts (steel columns)
VII. WArrAnty
Pico•Tag amino acid analysis column