Waters µStyragel Column - Manual (Page 8)

[ Care and Use ManUal ]
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
µStyragel Column 8
© 2008 Waters Corporation. Waters, The Science of What’s
Possible, µStyragel and UltraStyragel are trademarks of Waters
Corporation.
February 2008 WAT035957 Rev 3 VW-PDF
Sales Offices:
Austria and European Export
(Central South Eastern Europe,
CIS and Middle East) 431 877 18 07
Australia 2 9933 1777
Belgium 32 2 726 1000
Brazil 55 11 5094 3788
Canada 800 252 4752
China 8621 6495 6999
CIS/Russia +7 495 3367000
Czech Republic 42 02 617 11384
Denmark 45 46 59 8080
Finland +358 9 5659 6288
France (33) 1 30 48 72 00
Germany 49 6196 400600
Hong Kong 852 29 64 1800
Hungary 36 1 350 5086
India and India Subcontinent
91 80 2 837 1900
Ireland 353 1 448 1500
Italy 39 02 274 211
Japan (81) 3 3471 7191
Korea (82) 2 820 2700
Mexico 5255 5200 1860
The Netherlands +31 (0)76-50 87 200
Norway 47 63 84 60 50
Poland (48) 22 833 4400
Puerto Rico 787 747 8445
Singapore 65 6273 1221
Spain 34 93 600 93 00
Sweden 46 8 555 11500
Switzerland 41 62 889 2030
Taiwan 886 2 2543 1898
United Kingdom 44 208 238 6100
Column Type Test Sample (1-10 µl) µStyragel
100Å ODBC 4,000
500Å ODBC 3,000
103Å ODBC 3,000
104Å ODBC 3,000
105Å ODBC 3,000
106Å diclohexylphthalate (DCPH) or
C36H74 (hexatriacontane) 3,000
In plates, calculated by the tengent method. Calculating column efficiencies
by other methods, such as 5-sigma method, may give different values.
Elution distance from
injection point to the peak
apex on the chart paper.
Peak width of marker
measured where tangents
drawn on peak intersect
baseline (same unit as V).
Height
Inject
Pore Size Effective Molecular Weight Range Part #
100Å 50-1,500 WAT027167
500Å 100-10,000 WAT027161
103Å 200-30,000 WAT027166
104Å 5,000-600,000 WAT027165
105Å 50,000-4 million WAT027164
106Å 200,000->10 million WAT027163
Table 6. Recommended Values for Column Efficiency Test
Figure 7. Tangent Method for Calculating Column Efficiency Test
VI. ORDERING INFORMATION