Waters µStyragel Column - Manual (Page 6)

[ Care and Use ManUal ]
µStyragel Column 6
b. Storing the Column
If you will be using the column again in less than 24 hours, special
storage procedures are unnecessary. However, be sure that the
columns never dry out.
For longer storage periods, return the column to its box with the end
plugs firmly in place for storage. Do not leave a column at elevated
temperatures without solvent flow. Refer to Table 2 for information
about solvent compatibility for your column.
c. Efficiency Testing
Waters columns are tested for adherence to our specifications. Slight
variations may occur depending on:
• The type and condition of equipment used
• The nature of the sample
• Instrument settings
Waters recommends that you perform your own efficiency tests. To
test both your system and each column in the system, perform both of
these tests:
• Band spreading instrument test
• Column efficiency test
These 2 tests are described below on the following page.
When to Perform Efficiency Tests
Perform each time you add columns or otherwise change your effi-
ciency tests system. Then, as you use the system, conduct efficiency
tests on a regular schedule.
If problems occur during normal operation of the column, repeat the
conditions for the initial efficiency tests and compare the results.
Differences in the results may help you to identify the source of the
problem.
Monitor band spreading due to system components frequently by
performing a column efficiency test without the column in line. Be
sure to resolve the cause of excessive band spreading before attach-
ing columns to the system.
Problem Cause Corrective Action Prevention
Buildup in system operating
pressure Inlet filter insert (from the first column
only) plugged with particulates. Check
for injector and pump seal shedding. Replace the filter insert Use a Styragel Guard Column
or fit an in-line filter between
the pump and the first column.
Clogged tubing Replace the tubing Filter the sample.
Loss of resolution, broad peaks,
low plate counts Filter inserts partially blocked Replace or clean inlet and/
or outlet filter inserts Always filter samples and
solvents.
Failing injector Repair the injector Filter solvents and use an
in-line filter.
Insufficient equilibration Continue equilibration Always equilibrate system until
you have a stable baseline.
Column damaged Replace column Use care in changing solvents
and flow rates.
Table 5: Column Troubleshooting
V. CaRe anD maIntenanCe
a. Troubleshooting
Table 5 describes specific problems, corrective actions, and preventive measures.