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Care and use manual – Waters BioSuite Peptide Analysis Columns User Manual

Page 5

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[ Care and Use ManUal ]

BioSuite Peptide Analysis Columns

5

In a proper tubing/column connection (Figure 2), the tubing touches the
bottom of the column endfitting, with no void between them.

Tubing touches the bottom of the

column endfitting, with no void between them.

Figure 2: Proper Tubing / Column Connection

The presence of a void in the flow stream reduces column performance.
This can occur if a Parker ferrule is connected to a Waters style endfitting
(Figure 3).

A void appears if the tubing with a Parker

ferrule is connected to a Waters style column.

Figure 3: Parker Ferrule in a Waters Style Endfitting

There is only one way to fix this problem: Cut the end of the tubing with the
Parker ferrule, place a new Waters ferrule on the tubing and make a new con-
nection. Before tightening the screw, make sure that the tubing bottoms out
in the endfitting of the column. Conversely, if tubing with a Waters ferrule is
connected to a column with Parker style endfitting, the end of the tubing will
bottom out before the ferrule reaches its proper sealing position. This will
leave a gap and create a leak (Figure 4).

The connection leaks if a Waters ferrule is

connected to a column with a Parker style endfitting.

Figure 4: Waters Ferrule in a Parker Style Endfitting

There are two ways to fix the problem:

1. Tighten the screw a bit more. The ferrule moves forward, and reaches

the sealing surface. Do not over tighten since this may end in breaking
the screw.

2. Cut the tubing, replace the ferrule and make a new connection.

Alternatively, replace the conventional compression screw fitting with
an all-in-one PEEK fitting (Waters part number PSL613315) that allows
resetting of the ferrule depth. Another approach is to use a Keystone,
Inc. SLIPFREE

®

connector to always ensure the correct fit. The finger tight

SLIPFREE connectors automatically adjust to fit all compression screw type
fittings without the use of tools, (Figure 5).

Figure 5: Single and Double SLIPFREE Connectors

Features:

Tubing pushed into endfitting, thereby guaranteeing a void-free

connection

• Connector(s) come(s) installed on tubing

Various tubing i.d. and lengths available

• Finger tight to 10,000 psi – Never needs wrenches

• Readjusts to all column end fittings

• Compatible with all commercially available end fittings

Unique design separates tube-holding function from sealing

function

Table 2. Waters Part Numbers for SLIPFREE Connectors

SLIPFREE Type
and Tubing
Length

0.005”

Tubing Internal

Diameter

0.010”

0.020”

Single 6 cm

PSL 618000

PSL 618006

PSL 618012

Single 10 cm

PSL 618002

PSL 618008

PSL 618014

Single 20 cm

PSL 618004

PSL 618010

PSL 618016

Double 6 cm

PSL 618001

PSL 618007

PSL 618013

Double 10 cm

PSL 618003

PSL 618009

PSL 618015

Double 20 cm

PSL 618005

PSL 618001

PSL 618017

Void

Gap