Waters BioSuite Peptide Analysis Columns - Manual (Page 5)

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BioSuite Peptide Analysis Columns 5
In a proper tubing/column connection (Figure 2), the tubing touches the
bottom of the column endfitting, with no void between them.
Tubing touches the bottom of the
column endfitting, with no void between them.
Figure 2: Proper Tubing / Column Connection
The presence of a void in the flow stream reduces column performance.
This can occur if a Parker ferrule is connected to a Waters style endfitting
(Figure 3).
A void appears if the tubing with a Parker
ferrule is connected to a Waters style column.
Figure 3: Parker Ferrule in a Waters Style Endfitting
There is only one way to fix this problem: Cut the end of the tubing with the
Parker ferrule, place a new Waters ferrule on the tubing and make a new con-
nection. Before tightening the screw, make sure that the tubing bottoms out
in the endfitting of the column. Conversely, if tubing with a Waters ferrule is
connected to a column with Parker style endfitting, the end of the tubing will
bottom out before the ferrule reaches its proper sealing position. This will
leave a gap and create a leak (Figure 4).
The connection leaks if a Waters ferrule is
connected to a column with a Parker style endfitting.
Figure 4: Waters Ferrule in a Parker Style Endfitting
There are two ways to fix the problem:
1. Tighten the screw a bit more. The ferrule moves forward, and reaches
the sealing surface. Do not over tighten since this may end in breaking
the screw.
2. Cut the tubing, replace the ferrule and make a new connection.
Alternatively, replace the conventional compression screw fitting with
an all-in-one PEEK fitting (Waters part number PSL613315) that allows
resetting of the ferrule depth. Another approach is to use a Keystone,
Inc. SLIPFREE
® connector to always ensure the correct fit. The finger tight
SLIPFREE connectors automatically adjust to fit all compression screw type
fittings without the use of tools, (Figure 5).
Figure 5: Single and Double SLIPFREE Connectors
Features:
• Tubing pushed into endfitting, thereby guaranteeing a void-free
connection
• Connector(s) come(s) installed on tubing
• Various tubing i.d. and lengths available
• Finger tight to 10,000 psi – Never needs wrenches
• Readjusts to all column end fittings
• Compatible with all commercially available end fittings
• Unique design separates tube-holding function from sealing
function
Table 2. Waters Part Numbers for SLIPFREE Connectors
SLIPFREE Type
and Tubing
Length
0.005”
Tubing Internal
Diameter
0.010” 0.020”
Single 6 cm PSL 618000 PSL 618006 PSL 618012
Single 10 cm PSL 618002 PSL 618008 PSL 618014
Single 20 cm PSL 618004 PSL 618010 PSL 618016
Double 6 cm PSL 618001 PSL 618007 PSL 618013
Double 10 cm PSL 618003 PSL 618009 PSL 618015
Double 20 cm PSL 618005 PSL 618001 PSL 618017
Void
Gap