Waters MassPREP E. coli Digestion Standard - Manual (Page 2)

E. Coli Digestion Standard 2
[ Care and Use ManUal ]
V. reConstItutIon of MPds e. ColI for analysIs wIth the
waters ProteIn exPressIon systeM usIng MICrosCale
(300 �m scale) hPlC
Two vials of MPDS E. coli are required to execute the Waters Protein
Expression System training module. The lyophilized powder in the first
vial should be resuspended in 50 µl of 0.1% formic acid. The resulting
solution in the first vial should be transferred, via pipette, to the sec-
ond vial, and used to resuspend the remaining lyophilized powder. Mix
capped vial by vortexing to insure all material is resuspended. Refer to
the Waters Protein Expression System Operator’s Guide for details on
use the of the MPDS E. coli mixture for system training purposes.
VI. safety and Msds InforMatIon
MPDS mixtures contain various non-hazardous peptides in powder
form. They are for laboratory use only; and solely intended for
purposes of scientific experimentation, analysis, research, or develop-
ment. The mixtures should be used only as directed and in accordance
with good laboratory practice. All Waters MassPREP Digestion Stan-
dards are non hazardous as defined by OSHA’s Hazard Communication
Standard, 29 CFR 1910.1200(c) and Appendix A and B, and are thus
exempt from the requirements of the Hazard Communication Standard,
including the requirement to supply MSDSs.
VII. MassPreP dIgestIon standard orderIng InforMatIon
Item Part Number Quantity
MassPREP Digestion Standard, Enolase 186002325 1
MassPREP Digestion Standard, Phosphorylase b 186002326 1
MassPREP Digestion Standard,
Bovine Hemoglobin 186002327 1
MassPREP Digestion Standard, Alcohol
Dehydrogenase 186002328 1
MassPREP Digestion Standard,
Bovine Serum Albumin 186002329 1
MassPREP Digestion Standard Kit
(one of each std.) 186002330 5
MassPREP Digestion Standard, Mix 1 186002865 1
MassPREP Digestion Standard, Mix 2 186002866 1