Care and use manual, Phosphopeptide standards 3 – Waters MassPREP Phosphopeptide Standards User Manual
Page 3
[ Care and Use ManUal ]
Phosphopeptide Standards
3
V. EXAMPLE SPECTRA
The following MALDI-MS spectra (positive reflectron) of Phosphopep-
tide Standard – Enolase (top) and Enolase Digest with Phosphopep-
tides Mix (bottom) mixed with MassPREP DHB matrix were obtained
on a Waters MALDI micro MX. Each spectrum (200 laser shots) was
processed by baseline subtraction.
The following postive mode LC-ESI-MS (TIC) spectra were obtained
using a Waters Alliance 2795 coupled to a Waters ZQ 4000 mass
spectrometer. A 20 pmol/μL solution in 0.02% trifluoroacetic acid
in water was prepared and 30 μL was injected on a Waters BioSuite™
C
18
3 μm, PA – A, 2.1 x 150 mm column.
VI. STORAGE AND STABILITY
It is recommended that the MassPREP™ Phosphopeptide Standards
vial remain in the foil pouch (in the dark) at room temperature until
use. Once reconstituted in appropriate solvents, the solution can be
used for one week when stored in the dark at 4 °C. For longer term
storage, the solution should be aliquoted into microcentrifuge tubes
and stored at –20 °C.
VII. RELATED ACCESSORIES
%
0
100
%
0
100
Ma ssPREP
™
Enolase Digest with Phosphopeptides Mix , 2 pmol
Ma ssPREP
™
Phosphopeptide
Standard-Enolase, 1p mol
733.4
775 .1
813.4
814.4
815.4
863.4
1359.1
1368.7
1370.7
1448 .7
1450.6
T18 1P
T19 1P
T43 1P
T43 2P
726.3
756.4
757.4
807.4
813.3
863.3
1057.5
116 1.5
1288.6
1368.6
1412.7
1488.5
864.4
TOF LD+
5.23e3
TOF LD+
6.04e3
Time
5.00
10.00
15.00
20.00
25.00
30.00
35.00
40.00
45.00
%
0
100
5.00
10.00
15.00
20.00
25.00
30.00
35.00
40.00
45.00
%
0
100
T19 1P
T18 1P
T43 2P
T43 1P
T18 1P
T43 2P
T19 1P and o ther peptides
T14 3 1P and othe r peptides
MassPREP
™
Phosphopeptide
Standard-Enolase, 6 00 pmol
MassPREP
™
Enolase Digest with
Phosphopeptides Mix , 300 pmol
%
0
100
%
0
100
Ma ssPREP
™
Enolase Digest with Phosphopeptides Mix , 2 pmol
Ma ssPREP
™
Phosphopeptide
Standard-Enolase, 1p mol
733.4
775 .1
813.4
814.4
815.4
863.4
1359.1
1368.7
1370.7
1448 .7
1450.6
T18 1P
T19 1P
T43 1P
T43 2P
726.3
756.4
757.4
807.4
813.3
863.3
1057.5
116 1.5
1288.6
1368.6
1412.7
1488.5
864.4
TOF LD+
5.23e3
TOF LD+
6.04e3
Time
5.00
10.00
15.00
20.00
25.00
30.00
35.00
40.00
45.00
%
0
100
5.00
10.00
15.00
20.00
25.00
30.00
35.00
40.00
45.00
%
0
100
T19 1P
T18 1P
T43 2P
T43 1P
T18 1P
T43 2P
T19 1P and o ther peptides
T14 3 1P and othe r peptides
MassPREP
™
Phosphopeptide
Standard-Enolase, 6 00 pmol
MassPREP
™
Enolase Digest with
Phosphopeptides Mix , 300 pmol
Related Product Descriptions
Part Number
MassPREP Digestion Standard Enolase (1 vial)
186002325
MassPREP Calibration Mix – MALDI Reflectron (1 vial)
186002819
MassPREP MALDI Matrix CHCA (5 vials/1 pk)
186002331
MassPREP MALDI Matrix DHB (5 vials/1 pk)
186002333
MassPREP MALDI Matrix THAP (5 vials/1 pk)
186002335
MALDI Plate Box - for plate storage
290000479
BioSuite C18 3μm, PA – A, 2.1 x 150 mm
186002427