Waters MassPREP Phosphopeptide Standards - Manual

[ Care and Use ManUal ]
Phosphopeptide Standards 1
Contents
I. IntroduCtIon
II. synthetIC enolase PhosPhoPePtIdes
III. reCommended usage
IV. samPle PreParatIon
V. examPle sPeCtra
VI. storage and stabIlIty
VII. related aCCessorIes
massPreP PhosPhoPePtIde standards
I. IntroduCtIon
Phosphorylated proteins and peptides are difficult to detect and
characterize by mass spectrometry due to their low abundance and
low ionization efficiency. A set of standard samples can aid in devel-
oping methods for optimized phosphopeptide detection. As a tool
for screening sample preparation and sample enrichment methods
for phosphopeptides, Waters offers MassPREP™ Phosphopeptide
Standards containing synthetic phosphopeptides based on modified
peptides from tryptic yeast enolase digest (SwissProt P00924).
Commonly used phosphorylated samples are ß-casein digests or
standards which contain only two phosphopeptides, both modified
atserine. The four modified enolase peptides in the mixture are either
singly phosphorylated at serine, threonine, or tyrosine or doubly
phosphorylated at serine.
II. synthetIC enolase PhosPhoPePtIdes
Peptide Sequence Isotopic mass,
[M+2H]2+
[M+H]+
T18 1P NVPLpY K 813.3912 407.1995
T19 1P HLADL pSK 863.4028 432.2053
T43 1P VNQIG pTLSES IK 1368.6776 684.8428
T43 2P VNQIG TLpSEpS IK 1448.6439 724.8259