Waters XSelect HSS HPLC Columns - Manual

[ Care and Use ManUal ]
XSelect HSS HPLC Columns 1
Contents
I. IntRoDUCtIon
II. ConeCtInG tHe CoLUMn to tHe HPLC sYsteM
a. Column Connection
b. Column Connectors and System Tubing Considerations
c. Band Spreading Minimization
d. Measuring System Band Spread Volume
e. Measuring Gradient Delay Volume
III. WateRs sMaLL PaRtICLe sIze (3.5 �m) CoLUMns
– Fast CHRoMatoGRaPHY
IV. CoLUMn eqUILIbRatIon
V. CoLUMn InstaLLatIon PRoCeDURe
VI. CoLUMn PeRFoRManCe VaLIDatIon
VII. InItIaL CoLUMn eFFICIenCY DeteRMInatIon
VIII. CoLUMn UsaGe
a. Guard Columns
b. Sample Preparation
c. Recommended pH Range
d. Solvents
e. Pressure
f. Temperature
g. Scaling Up/Down Isocratic Methods
IX. CoLUMn CLeanInG, ReGeneRatInG anD stoRaGe
a. Cleaning and Regenerating
b. Storage
X. tRoUbLesHootInG
I. IntRoDUCtIon
Thank you for choosing an XSelect ™ High Strength Silica (HSS) HPLC
column. The manufacture of XSelect HSS HPLC columns begins with
ultrapure reagents to control the chemical composition and purity of
the final product. XSelect HSS HPLC columns are manufactured in a
cGMP, ISO 9001:2000 certified plant with each step being conducted
within narrow tolerances. Every column is individually tested and
Certificates of Batch Analysis and a Performance Chromatogram are
provided with each column.
XSelect HSS HPLC columns are based on the same particle technol-
ogy and chemistries as ACQUITY UPLC
® HSS columns, thus enabling
seamless transferability between HPLC and UPLC ® separations.
XseLeCt HIGH stRenGtH sILICa (Hss) HPLC CoLUMns