Waters ACQUITY UPLC CSH Columns - Manual (Page 9)

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ACQUITY UPLC CSH Columns 9
b. Recommended Flow Rates and Backpressures for ACQUITY UPLC CSH Columns
Note: 1. ACQUITY UPLC CSH 1.7 µm particle reversed-phase columns
2. ACN/Aqueous gradient, Pmax at ~30% acetonitrile
3. Approximate maximum total system backpressure given
2.1 mm i.d. Columns (40 °C)
UPLC Linear Velocity
(mm/sec)
3 4 5 6
Column Dimensions
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
2.1 x 30 mm 0.45 3000 0.60 4100 0.75 5100 0.9 6100
2.1 x 50 mm 0.45 4800 0.60 6400 0.75 8000 0.9 9500
2.1 x 100 mm 0.45 9100 0.60 12100 0.75 15200 0.9 18200
2.1 x 150 mm 0.45 13400 0.60 17900 0.75 22400 0.9 26900
3.0 mm i.d. Columns (40 °C)
UPLC Linear Velocity
(mm/sec)
3 4 5 6
Column Dimensions
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
3.0 x 30 mm 0.9 3400 1.17 4400 1.53 5800 1.8 6800
3.0 x 50 mm 0.9 5100 1.17 6600 1.53 8700 1.8 10200
3.0 x 100 mm 0.9 9300 1.17 12100 1.53 15900 1.8 18700
3.0 x 150 mm 0.9 13600 1.17 17600 1.53 23100 1.8 27100
1.0 mm i.d. Columns (40 °C)
UPLC Linear Velocity
(mm/sec)
3 4 5 6
Column Dimensions
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
Flow Rate
(mL/min)
Backpressure
(psi)
1.0 x 50 mm 0.1 4300 0.13 5600 0.17 7400 0.2 8700
1.0 x 100 mm 0.1 8600 0.13 11200 0.17 14600 0.2 17200
1.0 x 150 mm 0.1 12800 0.13 16700 0.17 21800 0.2 25600