Waters ACQUITY UPLC CSH Columns - Manual

[ Care and Use ManUal ]
ACQUITY UPLC CSH Columns 1
Contents
I. GettInG started
a. Column Connectors
b. Column Installation
c. Column Equilibration
d. eCord Installation
e. Initial Column Efficiency Determination
f. VanGuard Pre-Columns
II. CoLUMn Use
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
III. CoLUMn CLeanInG, reGeneratInG and storaGe
a. Cleaning and Regeneration
b. Storage
IV. IntrodUCInG eCord InteLLIGent CHIP teCHnoLoGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
V. addItIonaL InForMatIon
a. Tips for Maximizing ACQUITY UPLC CSH Column Lifetimes
b. Recommended Flow Rates and Backpressures for
ACQUITY UPLC CSH Columns
ACQUITY UPLC CSH CoLUmnS
Thank you for choosing a Waters ACQUITY UPLC ® CSH ™ column.
ACQUITY UPLC CSH columns feature Waters Charged Surface
Hybrid (CSH) Technology which provides excellent peak shape,
high efficiency and loading capacity for basic compounds when
using acidic, low ionic strength mobile phases. This same particle
technology is used in the XSelect
™ CSH family of HPLC columns,
thus enabling seamless transferability between HPLC and UPLC ®
system platforms. The ACQUITY UPLC CSH packing materials were
designed specifically for use with the ACQUITY UPLC systems and
are manufactured in a cGMP, ISO 9001 certified manufacturing
facility using ultra pure reagents. Each batch of ACQUITY UPLC CSH
material is tested chromatographically with acidic, basic and neutral
analytes and the results are held to narrow specification ranges to
assure excellent, reproducible performance. Every column is individu-
ally tested and a Performance Chromatogram and Certificate of Batch
Analysis are provided on the eCord
™ intelligent chip.
ACQUITY UPLC CSH columns were designed and tested specifically
for use on ACQUITY UPLC systems. ACQUITY UPLC CSH columns will
exhibit maximum chromatographic performance and benefits ONLY
when used on holistically-designed ACQUITY UPLC systems since these
systems and columns were created and designed to operate together.
For these reasons, Waters cannot support the use of ACQUITY UPLC
columns on any system other than an ACQUITY UPLC system.