Waters nanoACQUITY UPLC 2G Trap Columns - Manual (Page 2)

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[ CARE AND USE MANUAL ]
nanoACQUITY UPLC 2G Trap Columns
Figure 1. Trap column on a nanoACQUITY UPLC single pump configuration.
In order to achieve the highest performance from your
nanoACQUITY UPLC 2G Trap Column investment, we strongly
suggest you observe the care and use guidelines detailed below.
II. FIT TINGS
Depending on your Waters nanoACQUITY UPLC System and
application needs, the nanoACQUITY UPLC 2G Trap Column will
utilize two of the connector fittings shown in Figure 2.
Analytical column nano Tee Trap column
Waste
Needle
Figure 2: nanoACQUITY UPLC 2G Trap Column (10,000 psi) M-detail and
V-detail connection fittings.
nanoACQUITY 10-32/V-Detail
(10,000 psi rated)
nanoACQUITY 6-40/M-Detail
(10,000 psi rated)
1/4” Gold-plated
compression screw
3/16” Gold-plated
compression screw
Two-piece ferrule
Double-crimped
stainless steel sleeve
Steel sleeve
One-piece ferrule
nanoACQUITY 10-32/V-Detail
(10,000 psi rated)
nanoACQUITY 6-40/M-Detail
(10,000 psi rated)
1/4” Gold-plated
compression screw
3/16” Gold-plated
compression screw
Two-piece ferrule
Double-crimped
stainless steel sleeve
Steel sleeve
One-piece ferrule
III. CONNECTING THE COLUMN TO THE UPLC SYSTEM
These procedures outline the steps necessary to install your
nanoACQUITY UPLC 2G Trap Column (10,000 psi) onto a
nanoACQUITY UPLC System. Connecting and installing your
purchased trap properly is essential to minimizing dead volumes
and maximizing chromatographic performance. Make sure that all
connected trap fittings are bottomed into all ports and tees.
1. Start the fitting into the port.
2. Grasp the end fitting and gently push it into the appropriate
nanoACQUITY UPLC tee, port, or valve. Note that bottoming of
the Trap tubing into the port minimizes system dead volume.
3. Tighten the fitting according to the recommendations in the
following table.
■ ■
If the V-Detail fitting is new and not yet installed, tighten
the fitting snug (finger-tight) plus one full turn (360 degrees)
using a 1/4-inch wrench.
■ ■
If the M-Detail fitting is new and not yet installed, tighten
the fitting snug (finger-tight) plus 1/2 full turn (180 degrees)
using a 3/16-inch wrench.
■ ■
If either V-Detail or M-Detail fitting has been installed
and removed once or more, tighten the fitting snug (finger-
tight) plus 1/6 turn (60 degrees) using the correct wrench as
detailed above. Proper 60 degree final tightening is used for
both the V-Detail and M-Detail fittings when they have been
previously used.
IV. COLUMN USAGE
a. Mobile Phases
Use only filtered and degassed mobile phases that are compatible
with the column packing material. Switch only between mutually
miscible mobile phases to prevent possible precipitation in the
nanoACQUITY UPLC System lines and/or within trap columns.
Note: the Symmetry
® C
18
, 5 µm, 2G Trap Column is shipped in 100%
Optima ™ Acetonitrile (Fisher Scientific).
b. Sample Considerations
The presence of particulates in the injected sample will greatly
affect trap column life. Whenever possible, inject only filtered or
microcentifuged (e.g., 10,000 psi for 5 minutes) samples contained
in eluent that are miscible with the separation mobile phases.