Waters iKey - Manual (Page 8)

8
[ CARE AND USE MANUAL ]
iKey
V. REMOVING T HE iK EY FROM T HE ionK EY SOURC E
CAUTION: Opening the locking lever on the ionKey system without first stopping flow and decompressing the fluidic pressure
can damage the iKey.
1. Stop the solvent flow and decompress the fluidic pressure on the iKey using one of these methods.
a. Press the STOP FLOW button on the front of the source.
b. In the Console, select Sample Manager from the system tree, and then click > Maintain > Remove iKey.
2. Make sure the mobile phase flow is completely stopped and that the system pressure has decompressed below 100 psi. If the solvent
is flowing, the PRESSURE LED is steady green. The PRESSURE LED blinks green as the system is decompressing. Wait until the light is
turned off completely. If an ikey is removed without first decompressing the system, a “Removed under pressure” error message will
appear, indicating potential damage to the iKey.
3. Rotate the lever clockwise 180 degrees to the vertical position with the unlock indicator in the 12 o’clock position.
4. Pull the iKey from the docking port.
5. Place the iKey in the protective sheath.
WARNING: The electrospray emitter at the front end of the iKey contains a very small metal tip. Like a needle, it can pierce skin.
WARNING: If the iKey had been run at high temperature, the metal rings near the fluidic ports on the side may be hot.
Avoid touching them.
VI. CARING FOR T HE iK EY
a. Cleaning and Regeneration
Changes in peak shape, peak splitting, shouldering peaks, shifts in retention, change in resolution or increasing backpressure may
indicate iKey contamination. Flush with a neat organic solvent to remove the non-polar contaminant(s), taking care not to precipitate any
buffered mobile phase components. If this flushing procedure does not solve the problem, purge the iKey with the following cleaning and
regeneration procedures.
Use a cleaning routine that matches the properties of the samples and stationary phase type and will solubilize the suspected contaminate.
Flush with 20 iKey volumes of solvent at an intermediate temperature of 40 °C. Return to the initial mobile phase conditions by reversing
the sequence.
Purge the iKey with a sequence of progressively more non-polar solvents (e.g., water, methanol, acetonitrile, isopropanol, etc.). If iKey
performance has not improved after regeneration/cleaning procedures, contact your local Waters representative for additional support.
If iKey performance has not improved after regeneration/cleaning procedures, contact your local Waters representative for
additional support.
Unlock indicator in 12 o’clock position
Lever in vertical position