Waters MassPREP Online Desalting Cartridge - Manual (Page 2)

3. Install the new MassPREP On-line Desalting Cartridge, noting
the flow arrow direction on the device. This should match the
flow arrow direction on the analytical column.
4. Reassemble the Sentry 2.1 x 10 mm Guard Column Holder.
Hand-tighten only.
5. Connect the 2.1 x 10 mm Guard Column Holder containing
the MassPREP On-line Desalting Cartridge onto HPLC or
LC/MS System using appropriate connectors (e.g., PEEK
™
tubing and connectors). Prior to use, the MassPREP On-line
Desalting Cartridge reversed-phase packing material must
be “wet” with organic solvent prior to equilibration with 95%
Eluent A/5% Eluent B. This can be accomplished by running
a “blank injection” (i.e., buffer injection followed by running
the separations gradient) or by washing the column with a
minimum of five column volumes of 50% Eluent B followed by
a ten column volume equilibration at 95% Eluent A/5% Eluent
B prior to sample injection. While equilibrating, check the
holder and all connections for leaks.
Figure 1: Sentry Guard Holder (P/N WAT097958) and MassPREP On-line
Desalting Cartridge (P/N 186002785).
III. RECOMMENDED LC /MS SYST EM
CONFIGURATION TO MINIMIZE MS SOURC E
CONTAMINATION
The presence of non-volatile salts (e.g., NaCl) can suppress protein
ionization and foul the ionization source of the mass spectometer.
It is therefore important to remove or significantly minimize the
introduction of these compounds into the mass analyzer. Figure
2 details an LC/MS configuration that can effectively be used to
load sample onto the column and elute salt to waste prior to valve
switching and protein elution into the mass analyzer.
Position 1: Sample Loading and Salt Elution to Waste
Position 1 (Sample Loading and Salt Elution to Waste)
1
2
3 4
5
6
HPLC
Pump
Mass
Spectrometer
Recirculation
Waste
Gradient
HPLC
MassPREP
On-Line
Desalting
Cartridge
Position 2: Sample Elution to Mass Spec
Position 1 (Sample Loading and Salt Elution to Waste)
1
2
3 4
5
6
HPLC
Pump
Mass
Spectrometer
Recirculation
Waste
Gradient
HPLC
MassPREP
On-Line
Desalting
Cartridge
Figure 2: LC/MS Valve Switching Configuration for use with MassPREP
On-line Desalting Cartridge.
2
[ CARE AND USE MANUAL ]
MassPREP On-Line Desalting Cartridge