Waters ACQUITY UPC2 Torus Columns - Manual (Page 5)

5
[ CARE AND USE MANUAL ]
ACQUITY UPC 2 Torus Columns Care & Use
e. Useable Flow-Rate Ranges and Expected ACQUITY UPC 2
System Pressures
Table 2 estimates maximum flow rate and system pressure which
can be achieved without over-pressuring the ACQUITY UPC
2
System. The ACQUITY UPC 2 System has a maximum flow rate of
4 mL/minute and maximum pressure of 6000 psi (~400 Bar).
The predicted flow rates and expected backpressure information
is based on the ACQUITY UPC
2 System BPR (Backpressure
Regulator) set at 1800 psi (~120 Bar).
f. Band Spreading Minimization
The ACQUITY UPC 2 System has been designed to minimize band
spreading. Deviation from Waters specified tubing could result
in deterioration of chromatographic performance due to band
spreading induced by inappropriate tubing i.d. Figure 2 shows the
influence of tubing internal diameter on system band spreading
and peak shape. As can be seen, the larger tubing diameter causes
excessive peak broadening and lower sensitivity.
Figure 2: Effect of connecting tubing on system.
IV. T ROUBLESHOOTING
1. One of the most common problems with regards to columns
is incorrect or insufficient priming of the co-solvent/modifier
pump. If no peaks are observed after an injection or unusually
long retention times are observed, check the priming of the
co-solvent/modifier pump first.
2. If you see frosting on the column at the inlet or outlet, tighten
the compression screw on that side. If tightening doesn’t work,
depressurize the system and the column, and then replace the
fitting that is not sealing correctly.
3. If you continue to see frosting on the column, turn off the CO
2
and vent the system. Allow the column to depressurize fully
before disconnecting the inlet or outlet. Please contact your
Waters representative for additional support.
V. COLUMN CLEANING, EFFECTS OF ADDITIV ES,
AGING, AND STORAGE
Changes in peak shape, peak splitting, shoulders on the
peak, shifts in retention, change in resolution or increasing
backpressure may indicate contamination of the column.
Flushing with high concentrations of organic solvent, taking care
not to precipitate buffers, is usually sufficient to remove the
contaminant. If the flushing procedure does not solve the
problem, purge the column using the following cleaning and
regeneration procedures.
Diluted/Distorted Sample Band
0.005 inches
0.020 inches
0.040 inches
Dimension
100% CO
2
60/40 CO
2
/MeOH
Predicted Max Flow Rate
(mL/min)
Predicted Max Pressure
(psi)
Predicted Max Flow Rate
(mL/min)
Predicted Max Pressure
(psi)
2.1 x 50 mm 3.45 5581 2.00 6011
2.1 x 75 mm 2.65 5970 1.35 5938
2.1 x 100 mm 2.05 5969 1.05 6060
2.1 x 150 mm 1.40 5951 0.70 6002
3.0 x 50 mm 4.00 4179 3.60 5946
3.0 x 75 mm 3.75 4897 2.60 5959
3.0 x 100 mm 3.45 5499 2.00 5919
3.0 x 150 mm 2.70 5977 1.40 6023
Table 2. Useable Flow Rate Ranges and Expected ACQUITY UPC 2 System Pressures.