Waters ACQUITY UPC2 Torus Columns - Manual (Page 3)

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[ CARE AND USE MANUAL ]
ACQUITY UPC 2 Torus Columns Care & Use
c. eCord Installation
The eCord button should be attached to the side of the column
heater module. The eCord button is magnetized and does not
require specific orientation.
d. Column Equilibration
ACQUITY UPC 2 Columns are shipped dry. Equilibrate the column
with a minimum of 10-column volumes of the mobile phase prior
to use. (Refer to Table 2 for a listing of empty column volumes.)
Thermal and chemical equilibration are equally important
chromatography. Ensure that the mobile phase is preheated before
entering the column to prevent a thermal miss-match. This can be
done by enabling active preheating on a UPC
2 ® instrument.
e. Initial Column Efficiency Determination
1. Perform an efficiency test on the column before using it.
Waters recommends using a suitable solute mixture to analyze
the column upon receipt. We recommend that weaker solvents
are selected as injection solvents. Peak distortion can occur
due to strong solvent effects, resulting in lower efficiency
values. (Methanol can be considered a strong solvent under
SFC conditions.) It is recommended to use Waters Quality
Control Reference Material (QCRM) (p/n 186007950)
which contains a mixture of standards specifically chosen
for the ACQUITY UPC
2 Trefoil and Torus Columns. For more
information, please review the ACQUITY UPC 2 Columns
Brochure (literature code 720004275EN).
ACQUITY UPC 2 Quality Control Reference Materials (QCRM)
Intended Use Contents
Provides
convergence
chromatographic
performance
information for
both chiral and
achiral modes 1. 0.50 mg/mL (+/-) trans-Stilbene oxide
2. 0.50 mg/mL Thymine
3. 0.50 mg/mL Sulfamethoxazole
4. 0.50 mg/mL Sulfamethizole
In a 1 mL solution of 75:25 ACN:MeOH
2. Determine the number of theoretical plates (N) and use this
value as a benchmark for periodic comparisons.
3. Repeat the test at predetermined intervals to track column
performance over time. (Slight variations may occur
if performance tests are performed on two different
ACQUITY UPC
2 Systems due to the quality of the
connections, operating environment, system electronics,
reagent quality and column condition.)
Empty Column
Volumes (mL)
Column Internal Diameter (mm)
Column Length 2.1 mm 3.0 mm
50 mm 0.2 0.4
75 mm 0.3 0.6
100 mm 0.4 0.8
150 mm 0.5 1.0
Table 1. Empty Column Volumes in mL (multiply by 10 for f lush
solvent volumes).
f. VanGuard Pre-Columns
VanGuard™ Pre-columns are 2.1 mm i.d. x 5 mm length guard
column devices designed specifically for use on the ACQUITY
UPC
2 Systems. VanGuard Pre-columns are packed with the same
chemistries and frits as our 2.1 mm i.d. ACQUITY UPC 2 Columns.
VanGuard Pre-columns are designed to be attached directly to the
inlet side of an ACQUITY UPC
2 Column.
Note: In order to ensure void-free and leak-free connections, the
VanGuard Pre-column is shipped with the collet and ferrule NOT
permanently attached. Care must be taken when removing the
O-ring that holds these two pieces on the pre-column tubing.
Figure 1. Connecting a VanGuard Pre-column to an ACQUITY UPC
2 Column.
Installation Instructions
1. Remove VanGuard Pre-column from box and shipping tube and
remove plastic plug.
2. Orient pre-column so that male end is facing up and
carefully remove rubber O-ring that holds collet and ferrule
in place during shipping (collet and ferrule are not yet
permanently attached).
3. Orient ACQUITY UPC 2 Column perpendicular to work surface
so that column inlet is on the bottom (facing down), with
column outlet on top (facing up).
ACQUITY UPC 2 Column VanGuard Pre-Column
Place wrench here
Ferrule
Collet
Place wrench here
Flow