YSI BODAnalyst - Manual (Page 12)

Samples Section 2
YSI Incorporated BOD Analyst for Windows Software 8
Dilution Setup: You can have up to 5 dilutions for any Sample. On the General Setup Form, the
dilution unit field allows you to choose to express your dilutions in milliliters (ml) or in percent
(%). If you change dilution units on the General Setup Form, all of the dilutions in each of your
Samples will be changed automatically to reflect the new units. Dilutions can have up to two
places after the decimal point and three before it, however, on the YSI 5000/5100 Instrument
display, more characters for the dilution may mean fewer characters for the Sample name. So, try
to keep your dilutions to 3 or 4 characters or less (e.g. 300, 120, 75, 2.5).
Seed control sample name: This field is used to indicate the name of the seed control sample.
Simply choose the sample to be used as Seed control from the pull down list. Choose ‘No
Seeding’ from the pull down list for Samples that are not seeded. Only Samples that have the
Used as Seed field selected will appear on the list. Note that you will have to define your seed
control samples before you can define any seeded Samples. Often a plant's effluent is seeded
with material from the plant's influent. First define a Sample called "Influent." When you are
ready to define a Sample for the effluent, the seed control sample, "Influent" will be available for
selection. The next section is devoted to the topic of seeding.
Seed Qty in ml: Sets the quantity of seed material used for this Sample. The interpretation of
this field depends on the seeding method that you have selected on the General Setup Form. If
you have chosen to add seed directly to each bottle, then the number in this field represents the
number of milliliters of seed material in each seeded bottle. If you have chosen to add seed
material to the dilution water, then the number represents the number of milliliters of seed
material in each liter of the dilution water that is used for seeded Samples. In this case the field
will appear as Qty (ml/L):.
Standard: Treat sample as a GGA standard test. Standard tests have the Minimum and
Maximum Standard Test criteria applied to the tests.
Dilution Blank: Treat sample as a dilution blank test. Only the depletion is calculated for these
tests. The Maximum Dilution Blank Oxygen Depletion criteria is applied to Dilution Blank
Tests
Used as Seed: The sample can be used as a seed. With Used as Seed checked, a seed correction
will be calculated for the sample.
CBOD: If the Sample is a CBOD, check the box. This has absolutely no affect on the
calculations. It is only so that CBOD can be indicated on reports.
Decimal Points: The number of Decimal Points the final BOD for the sample should be
calculated to. Default is 0.
Pre-dilution: (The pre-dilution option is only shown when the General Setup Form option is
checked). Very strong samples sometimes require dilutions that would be difficult to make in one
step. In these cases, a sizeable quantity of sample is first diluted to a ratio that will make the final
dilutions practical. In the BOD Analyst software, we call this ratio the pre-dilution. For example,
if you add 10ml of your dairy farm sample to a 1000ml volumetric flask and then fill the flask
with dilution water, we call that a pre-dilution of 100. It is sometimes referred to as a serial
dilution. Some people find it easier to adjust for the strength of each sample with the pre-dilution
while keeping the final dilutions constant for all samples. Seed control samples, and blanks
cannot be pre-diluted.