Waters Delta-Pak High Pressure Insert HPLC Column - Manual

[ Care and Use ManUal ]
1
I. IntroductIon
a. Overview
Waters High Pressure Inert (HPI) HPLC columns are a metal free alternative
for both bio-chromatographic and ion-chromatographic applications. In this
advanced design, the sample contacts only polyetherether ketone (PEEK
® )
and ultrahigh molecular weight polyethylene (UHMWPE), two materials
which are widely accepted for their biocompatibility and inertness. The
chromatographic performance of these columns is essentially identical
to their metal counterparts with the added advantage of greater chemical
inertness.
b. Delta-Pak Columns
The Delta-Pak TM column packing material is an endcapped C
18
or C
4
bonded
phase. It is synthesized from 5 µm spherical silica particles having an aver-
age pore diameter of 100 Å or 300 Å. Delta-Pak
columns are ideal for the
analysis and purification of peptides and proteins.
contents
I. IntroductIon
a. Overview
b. Delta-Pak Columns
II. InstallatIon
a. Installing the Column
b. Equilibration
III. MoBIle PHase and saMPle GuIdelInes
a. Solvent Compatibility
b. Mobile Phase Requirements
c. Sample Preparation and Filtration
IV. oPeratIon
a. Chromatography Guidelines
b. Efficiency Testing
c. Typical Column Backpressure and Eluent Viscosity
d. Column Temperature Limits
V. care and MaIntenance
a. Troubleshooting
b. Cleaning the Column
c. Storing the Column
VI. orderInG InforMatIon
delta-Pak HIGH-Pressure Inert HPlc coluMn