Waters RapiGest SF Surfactant - Manual (Page 3)

[ Care and Use ManUal ]
RapiGest SF Surfactant 3
VII. SuggeSted SaMple preparatIon for Hplc and MaSS
SpectroMetry
. Add TFA to the digested protein samples. The final TFA concen-
tration should be approx. 0.5% (pH < 2).
(Note: Obtain high purity Trifluoroacetic acid (TFa). The purchase and use
of sealed vials containing 1 mL of high purity TFa is recommended.)
2. Incubate the sample at 37 °C for 30 – 45 minutes. Slight cloudi-
ness should be observed.
3. Centrifuge acid treated samples at 3,000 rpm for 0 minutes.
The hydrolytic RapiGest SF by-products are water immiscible
therefore, some precipitation may be observed.
4. Carefully transfer the solution to another microcentrifuge tube or
HPLC sample vial.
5. Proceed with MALDI Tof, HPLC or LC/MS analysis.