Waters Glyco-Pak N Columns - Manual (Page 6)

[ Care and Use ManUal ]
Glyco-Pak N Columns 6
To aid in diagnosing potential column problems:
• Convert the column to acetonitrile/water (70:30) with a linear gradient
over 1 hour at 0.5 m1/min.
• Equilibrate the column for 1 hour in premixed (w/w) acetonitrile/water
(70:30) at a flow rate of 1 ml/min.
• Inject 10 µl of a 10 mg/ml solution of N-acetylglucosamine.
• Set detector to 214 nm.
Retention time will vary with absolute concentration of the solvents. The peak
shape, however, should be similar to that seen in the chromatogram below.
Figure 7: Test N-Acetylglucosamine Chromatogram
Performing this test prior to. discussing the problem with your Waters repre-
sentative will help in diagnosing the problem.
IV. maIntenance and troubleshootIng
The following sections explain how to store and troubleshoot the column.
a. Storage
Leaving the column unused for less than 72 hours does not generally require
storage procedures.
Store the column in mobile phase or an acetonitrile/water 70:30 mixture.
Return the column to its box with the end plugs firmly in place for storage.
Allowing columns to dry out can result in poor chromatographic perfor-
mance.
CAUTION: Glyco-Pak N columns are shipped in water. Ensure that the storage
temperature does not go below 4 °C. The column may be damaged at tem-
peratures below this value.
b. Troubleshooting
Use Table 2 to troubleshoot problems with your column.
Table 2: Column Problems and Solutions
V. appendIx
a. Warranty/Service Information
Waters Corporation staff of experienced service specialists provide mainte-
nance assistance on both preventative and/or corrective levels. For complete
information and assistance, please call Waters Service Department at 1-800-
252-HPLC. For solutions to particular applications questions Waters team of
technical support personnel are available to help you with specialized support.
They may be contacted at 1-800-252-HPLC in Milford, MA.
Waters Corporation warrants its high performance liquid chromatography
columns in accordance with the following terms and conditions:
Waters replace without cost any steel column that fails to perform satisfacto-
rily if notified within 90 days from your receipt. Any column returned must
have a Return Authorization Number granted by the waters Customer Service
Department. Approval is subject to the following exclusions:
• Physical damage to the column because of misuse or abuse.
• Chemical damage to the packing material because of use with incompat-
ible mobile phases or buffers, or at an incorrect pH.
• Physical damage to the packing material because of operation at incor-
rect temperatures or pressures.
• Particulate buildup or precipitation in the column or end fittings causing
high internal pressure which has occurred because of improper mobile
phase or sample filtration practices.
Problem Cause Solution
Excess pressure
buildup
• Filters plugged
with Particulates
• Sample pre-
cipitates on column
(sample not soluble
in mobile phase)
• Clean in an ultrasonic bath
or replace.
• Always alter mobile phases
and samples.
• Slowly purge with a strong
mobile phase that is both
appropriate to dissolve the
contaminate and compatible
with the column.
Loss of resolution,
broad peaks, low
plate counts
• Contaminated
column
• Insufficient
equilibration
• Filters partially
plugged
• Slowly purge with very
strong mobile phase.
• Continue equilibration
• Replace or clean inlet and
outlet filters in an ultrasonic
bath.