Waters Oasis Glass Cartridges - Manual

[ Care and Use ManUal ]
Oasis HLB Glass Cartridges 1
Contents
I. IntroduCtIon
II. QuICk start sPe ProCedure
III. adjustments to oPtImIze reCoverIes
Iv. PreParatIon of PhosPhate-Buffered salIne (PBs)
a. Sample Comminution
b. Extraction/Partitioning
c. Dispersive Solid-Phase Extraction
v. orderIng InformatIon
a. Extraction Solution Preparation
oasIs hlB glass CartrIdges
I. IntroduCtIon
Waters Oasis ® HLB glass cartridges are available in 5 cc (200 mg)
configuration with Teflon ® frits. The clean glass cartridge is designed
for trace analysis at parts per trillion level including monitoring
endocrine disruptors, such as phenols and phthalates. The Oasis
glass cartridge contains a unique (patented
1 ) sorbent, a copolymer
designed to have a hydrophilic-lipophilic balance (HLB), that gives
high and reproducible recoveries for acidic, basic, and neutral com-
pounds - even if the cartridge runs dry.
Each lot of glass cartridges and Teflon frits are tested for the
presence of bisphenol A and other phenols and phthalates before
packing. These tests assure that endocrine disruptors, in water
samples, can be analyzed to part per trillion levels.
This Instruction Sheet outlines in Section II the Quick Start Solid-
Phase Extraction (SPE) Procedure for extracting endocrine disruptors
and many other types of compounds. Section III gives a simple
protocol to determine if any adjustments are necessary to optimize
recovery. Section IV describes the preparation of phosphate-buffered
saline (PBS) solution (required only for serum, plasma or urine
matrix).
The Certificate of Analysis (COA) reports recoveries, with RSDs, for
three polar pharmaceutical compounds. The COA displays results
from stringent quality control tests on the batch of polymer sorbent
and the lot of packed cartridges.
1 US Patent # 5,882,521