Waters Sirocco Protein Precipitation Plate - Manual

[ Care and Use ManUal ]
1 Sirocco Protein Precipitation Plate
Sirocco Protein PreciPitation Plate
The Sirocco ™ plate was designed to improve the automation and through-
put of protein precipitation in drug metabolism/ADME/toxicology labs.
The Sirocco Protein Precipitation Plate is a unique combination of novel
96-well plate tip design with proprietary new filter membranes.
A straightforward 7-step procedure designed to enable precipitate free
sample filtrates suitable for high-throughput LC/MS analysis.
Step 1 – Preparation
Place the Sirocco Plate on a collection plate for processing. This protects
the valve tips during the processing steps.
Sirocco Protein
Precipitation Plate
96-Well Collection Plate
Step 2 – Add Crash Solvent
Add precipitation solvent to all wells. Use a ratio of at least 3:1 solvent
to sample. Acetonitrile is the recommended precipitation solvent. The
Sirocco plate is designed to prevent solvent from leaking through the
plate at this step.
Step 3 – Load Sample
Add 20-200 µL of sample (plasma, urine, etc.) rapidly into the wells. Rapid
delivery of sample into the crash solvent creates mixing and initiates
precipitation process.