Waters High Strength Silica Columns - Manual

[ Care and Use ManUal ]
HSS HPLC Columns 1
Contents
I. IntRoDUCtIon
II. ConeCtInG tHe CoLUMn to tHe HPLC sYsteM
III. WateRs sMaLL PaRtICLe sIze (3.5 �M) CoLUMns
– Fast CHRoMatoGRaPHY
IV. CoLUMn eqUILIbRatIon
V. CoLUMn InstaLLatIon PRoCeDURe
VI. CoLUMn PeRFoRManCe VaLIDatIon
VII. InItIaL CoLUMn eFICIenCY DeteRMInatIon
VIII. CoLUMn UsaGe
IX. CoLUMn CLeanInG, ReGeneRatInG anD stoRaGe
X. tRoUbLesHootInG
I. Int RoDUCtIon
Thank you for choosing a High Strength Silica (HSS) HPLC column.
The manufacture of HSS HPLC columns begins with ultrapure
reagents to control the chemical composition and purity of the
final product. HSS HPLC columns are manufactured in a cGMP,
ISO 9001:2000 certified plant with each step being conducted
within narrow tolerances. Every column is individually tested and
Certificates of Batch Analysis and a Performance Chromatogram are
provided with each column.
HSS HPLC columns are based on the same particle technology and
chemistries as ACQUITY UPLC HSS columns, thus enabling seamless
transferability between HPLC and UPLC separations.
Waters recommends the use of Sentry ™ guard columns to maximize
column lifetime and protect the column from contaminants.
HIGH stRenGtH sILICa HPLC CoLUMns