Waters PIC Reagents - Manual (Page 2)

[ Care and Use ManUal ]
PIC Reagents 2
[ Care and Use ManUal ]
Column: µBondapak C
18
, 3.9 x 300 mm steel column
Part Number: WAT027324
Mobile Phase: Methanol/Water 50:50 with PIC Reagent
Flow Rate: 2.0 ml./min
Detection: 254 nm
Sample: 1. Niacinamide
2. Pyridoxine
3. Thiamine
4. Riboflavin
PIC Separation of Water-Soluble B Vitamins
Table 2 suggests the PIC reagent to use to separate the compo-
nents of some typical compounds on a C18 column with a water/
methanol eluent.
Table 2. Reagents to Use for Common Compounds
IV. usIng and storIng Waters PIC reagents
Waters PIC Reagents are premixed and buffered. Add 1 vial of reagent
to 1 L of mobile phase and filter.
Note: Crystals may form when vials are exposed to lower temperatures.
If crystals have formed, gently warm vial until crystals go back into
solution.
Store unopened reagent for up to one year at room temperature.
Compound or Class Reagent Compound or Class Reagent
Alkaloids Antitussives B
Cephaeline B Vitamins B5/B7
Emetinee B Calcium Leucovorin A
Ergot alkaloids B FD&C Dyes A
Analgesics/Antipyretics Folic Acid A
APC A Germicides
Aspirin A Benzalkonium Chloride B
Codeine B Cetylpyridinium Chloride B
Indomethacin A Imides A
Meperidine B Methotrexate A
Methadone B Petroleum Sulfonates A
Morphine B Surfactants
Propoxyphene B Quaternary amines B
Antibiotics Sulfonates A
Cephalosporins A or B
Sympathomimetics
(Decongestants)
Penicillins A or B Amphetamine B
Antihistamines Ephedrine B
Chlorpheniramine B Epinephrine B
Diphenhydramine B Isoproterenol B
Methapyrilene B Naphazoline B
Phenindamine B Nylidrin B
Pyrilamine B Phenylephrine B
Tripelennamine B Phenylpropanolamine B
Pheniramine B