Waters Ostro Sample Preparation Products - Manual

[ Care and Use ManUal ]
1 Ostro Sample Preparation Plate
Contents
I. INTRODUCTION
II. PROCeDURe
III. ORDeRINg INfORmaTION
I. INTRODUCTION
Ostro ™ sample preparation plates provide a novel approach for the
removal of phospholipids from biological samples. The Ostro 96-well
plate design uses an in-well protein precipitation in combination
with a single, rapid, pass-through method that provides consistent,
high extraction recoveries for acids, bases, and neutrals. Designed
specifically to meet the needs of the bioanalytical scientist, Ostro
enhances a laboratory’s performance by providing greater repro-
ducibility, improved recovery, and increased phospholipid
removal when compared with competitive techniques.
A straightforward 6-step procedure was designed to facilitate in-well
protein precipitation and produce filtrates free of proteins and other
endogenous components. These filtrates are then suitable for high-
throughput LC/MS/MS analysis.
II. PROCeDURe
Step 1: Preparation
Place the Ostro plate onto a 2 mL collection plate for processing.
Step 2: Plasma Loading
Add between 50 and 200 μL of plasma sample into the wells. The
Ostro plate is designed to prevent plasma samples from leaking
through the plate.
Step 3: Precipitation
Solvent Loading
Forcefully/rapidly add 1% HCOOH (formic acid) in acetonitrile to the
wells in a ratio of 3:1 solvent to plasma.
Note: The forceful/rapid addition of the solvent aids in mixing the
plasma with this solvent.
Note: Take care to ensure that all of the pipette tips are properly
positioned in the samples well to prevent any loss of mixing of samples.
ostro sample preparation plate
96-Well 2 mL Collection Plate
Ostro Plate