Waters Neutrals Quality Control Reference Materials - Manual (Page 3)

Neutrals Quality Control Reference Material 3
[ Care and Use ManUal ]
The table below indicated the approximate retention times obtained
for the compounds when using the specified chromatographic method
in Figure 1.
Table 1: Neutrals QCRM.
Column: BEH C
18
1.7 µm; 2.1 x 50 mm
Instrument: ACQUITY UPLC ® H-Class
Wavelength: 254 nm
Mobile Phases: A: 20 mM Potassium Phosphate pH 7
B: 10 mM Ammonium Formate pH 3
C: 10 mM Ammonium Bicarbonate pH 10
D: Acetonitrile
Flow Rate: 0.5 mL/minute
Temperature: 30 °C
Injection Volume: 1 μL
Figure 1 shows an example of the chromatography obtained for the
Neutrals QCRM mixture via UV at 254 nm, when the methods above
are used with a BEH C
18
, 1.7 µm, 2.1 x 50 mm.
Neutral compounds should not move with pH change!
10 mM Ammonium Formate pH 3; Acetonitrile
(50/50)
20 mM Potassium Phosphate pH 7; Acetonitrile
(50/50)
10 mM Ammonium Bicarbonate pH 10; Acetonitrile
(50/50)
1. Acetone (Vo) 2. Naphthalene 3. Acenaphthene
1
2
3
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 min
Figure 1. Chromatographic Separation via UV BEH C
18
, 1.7 µm,
2.1 x 50 mm.
The standards are manufactured in our ISO 9001, ISO 17025 facility.
Each standard is manufactured to ensure optimal reproducibility from
lot-to-lot. A Waters QCRM can be depended on for its’ accuracy.
This removes one variable from your system variability and provides
you the most dependable starting point for your testing.
If the QCRM box shows significant damage, notify the carrier and
your supplier at once and retain evidence of shipping damage so
that a claim can be made.
IV. orderIng InforMatIon
Order Neutrals QCRM 1860006360 for use with any of the column
products listed on the following pages.
Compound
Approximate RT (min)
(BEH C
18
, 5 µm, 2.1 50 mm, 0.5 mL/min)
Acetone (V0) 0.3
Naphthalene 1.5
Acenaphthene 2.6
Description Part Number
Neutrals Quality Control
Reference Material
1860006360