Waters System Evaluation Matrix 1 - Manual (Page 2)

System Evaluation Matrix #1 2
[ CARE AND USE MANUAL ]
III. Reconstitution of the System Evaluation Matrix #1
1. The vial contains 33.33 mg of lyophilized matrix. Add 1 mL of
water to the vial to make a concentration of 33.33 mg/mL.
2. Recap and then agitate the vial contents (e.g. via vortexing or
ultrasonic bath), to ensure the lyophilized powder dissolves.
3. For use on SYNAPT ® G2-S and detectors of similar sensitivity
dilute 1:20 in water.
IV. Use with an Analytical Standard
The matrix provides an abundance of peaks. By adding an analytical
standard the matrix can be used as a background.
Waters Analgesic Mix (P/N 186006350) can be added to provide
a collection of small molecules suitable for metabolomics method
development. Refer to note 6 in section VII.
• Prepare a 200 ng/µL stock by adding 1 mL acetonitrile to the
Analgesic mix vial.
• Prepare 50 ng/µL working concentration by taking 25 µL of the
stock and diluting with 75 µL of water.
• Add 5 µL of 50 ng/µL Analgesic Mix to 495 µL of the matrix to
make a final concentration of 0.5 ng/µL.
V. Example of Using the System Evaluation Matrix #1 and
Data Aquisition
Figure 1 shows a representative chromatogram of the control matrix
diluted 1:20 on a SYNAPT G2-S.
VI. ORDERING INFORMATION
To order these products, contact your nearest subsidiary, or visit
www. waters.com and click on Order Center.
Description Part number
System Evaluation Matrix #1 186006962
Analgesic Mix 186006350
VII. Additional Resources
1 ACQUITY UPLC ® I-Class Documentation CD (715003160)
2 Symmetry ® Columns Care and Use Manual (WAT047278).
3 SYNAPT G2-S HDMS online Help (access from the Waters MassLynx ® software).
4 MassLynx online Help (access from the Waters MassLynx software).
5 ACQUITY UPLC I-Class System online Help (access from Waters MassLynx software).
6 TransOmics ™ Informatics for Metabolomics and Lipidomics User Guide
For additional, detailed information, refer to the Waters Omics Research Platform Solution for
Metabolomics and Lipidomics System Guide (715003815).
You can also consult the Waters Web site, www.waters.com/omics.
Figure 1. Analysis of Metabolites in System Evaluation Matrix #1.
UPLC Conditions:
Column:
ACQUITY UPLC HSS T3, 1.8 μm, 2.1 x 100 mm
Column Temp.: 40 °C
Sample Temp.: 6 °C
Flow Rate: 500 µL/min
Injection Vol.: 2 µL, 6 s post injection strong wash
Run Time: 12 mins
Solvent A: water/0.1% formic acid
Solvent B: acetonitrile/0.1% formic acid
Wash: water/0.1% formic acid
Seal Wash: 50:50 water/methanol
Source Parameters:
Tune for maximum sensitivity
Capillary: 3.5 kV
Sampling cone: 25 V
Source temperature: 120 °C
Desolvation temperature: 500 °C
Desolvation/Nebulizer/Cone gas: 1000/6/50 L/hr
Source offset: 80 V
LockSpray Solution: 0.2 ng/µL leucine enkephalin in 50% methanol/50% water + 0.1% formic acid (556.2771 Da)
MS Method Parameters:
Tune for maximum sensitivity
Ionization mode: ESI+
TOF acquisition mode: Resolution
Acquisition method: Continuum MS E
TOF mass range: 50-1200 Da
Scan time: 0.1 s
Collision energy function 2: Trap CE ramp 20 to 40 V
Gradient
Time (min)
%A %B Curve
0.0 99 1 Initial
1.0 99 1 6
3.0 85 15 6
6.0 50 50 6
9.0 5 95 6
10.0 5 95 6
10.1 99 1 6