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Care and use manual, Waters corporation, Additional consumables – Waters GlycoWorks High-throughput Sample Preparation Kit User Manual

Page 6: Glycan sample preparation kit and standards

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[ CARE AND USE MANUAL ]

Waters Corporation

34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com

©2013 Waters Corporation. Waters and The Science of What's
Possible are registered trademarks of Waters Corporation.
GlycoWorks, and RapiGest are trademarks of Waters Corporation.

715004079 September 2013 Rev D SC-PDF

Description

Part No.

GlycoWorks HILIC 1 cc
Flangeless Catridges Pack

20/pack

186007239

Dextran Calibration Ladder

200 µg/vial

186006841

Glycan Performance Test Standard

228 pmol total/vial

186006349

RapiGest SF

1 mg vial/5 pack

186001861

96-well Collection Plate

96 wells/50 pack

186002481

Cap Mats for 1 mL Collection Plate

50/pack

186002483

µElution Plate Manifold

186001831

SPE Vacuum Pump

115, 60 Hz

176002986

Positive Pressure Manifold

186006961

Additional Consumables

C

Calibration

P

Performance Check

Glycan Sample Preparation Kit and Standards

Description

Part No.

GlycoWorks High-throughput Prep Kit

GlycoWorks HILIC µElution Plate, 96-well

RapiGest SF 1 mg Vial

GlycoWorks Control Standard, 100 µg Vial

GlycoWorks Reagent Kit
Manifold Waste Tray

176003090

186002780

186001860

186007033

186007034
600001282

GlycoWorks Single Use Prep Kit

GlycoWorks HILIC 1 cc Cartridge (20/pk)

RapiGest SF 1 mg Vial

GlycoWorks Control Standard, 100 μg Vial

GlycoWorks Reagent Kit

176003119

186007080

186001860

186007033

186007034

Glycan Performance Test Standard

The Glycan Performance Test Standard is a 2-AB labeled
human IgG-like standard that is QC verified to contain
the components needed to benchmark and evaluate
ACQUITY UPLC BEH Glycan, 1.7 µm Columns.

186006349

Dextran Calibration Ladder

The 2-AB labeled, Dextran Calibration Ladder is used
to calibrate the HILIC column from retention time to GU
values. This calibration ladder provides good peak shape
and reliable identification from 2 to 30 Glucose Units.

186006841

GlycoWorks HILIC 1 cc Flangeless Catridges Pack

This pack of 20 catridges, good for 10 analyses, are
offered for those customers who prefer to use the single
use devices with the positive pressure manifold (PPM).

186007239

VI. ORDERING INFORMATION

Glycan Analysis System Standards and Mobile Phases

Description Usage

Volume

Part No.

Alliance with Fluorescence

C

P

Qualification Standards Test Kit

700002753

Fluorescence Detector

P

Standard Solution

5.0 pg/µL anthracene in
20:80 water/acetonitrile

1 mL

700003694

Fluorescence Detector
Performance

P

Standard Solution

0.10 mg/L anthracene in
70:30 acetonitrile/water

10 mL

WAT047685

Ammonium Formate Concentrate

5000 mM Ammonium Formate in a
Water w/ 3.8% Formic acid matrix.
This allows the end user to dilute the
concentrate 10 mL to 1 L before using to
reach a 50 mM concentration.

10 mL

186007081

Kit contains seven 10 mL vials of varying concentrations of anthracene in
80:20 acetonitrile/water:

(1) 0.5 pg/μL

(1) 1.0 pg/μL

(2) 5.0 pg/μL

(1) 10.0 pg/μL

(1) 2.5 ng/mL

(1) 2.5 μg/mL

One blank 10 mL vial of 80:20 acetonitrile/H

2

0