Care and use manual, Waters corporation, Additional consumables – Waters GlycoWorks High-throughput Sample Preparation Kit User Manual
Page 6: Glycan sample preparation kit and standards
[ CARE AND USE MANUAL ]
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
©2013 Waters Corporation. Waters and The Science of What's
Possible are registered trademarks of Waters Corporation.
GlycoWorks, and RapiGest are trademarks of Waters Corporation.
715004079 September 2013 Rev D SC-PDF
Description
Part No.
GlycoWorks HILIC 1 cc
Flangeless Catridges Pack
20/pack
186007239
Dextran Calibration Ladder
200 µg/vial
186006841
Glycan Performance Test Standard
228 pmol total/vial
186006349
RapiGest SF
1 mg vial/5 pack
186001861
96-well Collection Plate
96 wells/50 pack
186002481
Cap Mats for 1 mL Collection Plate
50/pack
186002483
µElution Plate Manifold
–
186001831
SPE Vacuum Pump
115, 60 Hz
176002986
Positive Pressure Manifold
–
186006961
Additional Consumables
C
Calibration
P
Performance Check
Glycan Sample Preparation Kit and Standards
Description
Part No.
GlycoWorks High-throughput Prep Kit
GlycoWorks HILIC µElution Plate, 96-well
RapiGest SF 1 mg Vial
GlycoWorks Control Standard, 100 µg Vial
GlycoWorks Reagent Kit
Manifold Waste Tray
176003090
186002780
186001860
186007033
186007034
600001282
GlycoWorks Single Use Prep Kit
GlycoWorks HILIC 1 cc Cartridge (20/pk)
RapiGest SF 1 mg Vial
GlycoWorks Control Standard, 100 μg Vial
GlycoWorks Reagent Kit
176003119
186007080
186001860
186007033
186007034
Glycan Performance Test Standard
The Glycan Performance Test Standard is a 2-AB labeled
human IgG-like standard that is QC verified to contain
the components needed to benchmark and evaluate
ACQUITY UPLC BEH Glycan, 1.7 µm Columns.
186006349
Dextran Calibration Ladder
The 2-AB labeled, Dextran Calibration Ladder is used
to calibrate the HILIC column from retention time to GU
values. This calibration ladder provides good peak shape
and reliable identification from 2 to 30 Glucose Units.
186006841
GlycoWorks HILIC 1 cc Flangeless Catridges Pack
This pack of 20 catridges, good for 10 analyses, are
offered for those customers who prefer to use the single
use devices with the positive pressure manifold (PPM).
186007239
VI. ORDERING INFORMATION
Glycan Analysis System Standards and Mobile Phases
Description Usage
Volume
Part No.
Alliance with Fluorescence
C
P
Qualification Standards Test Kit
700002753
Fluorescence Detector
P
Standard Solution
5.0 pg/µL anthracene in
20:80 water/acetonitrile
1 mL
700003694
Fluorescence Detector
Performance
P
Standard Solution
0.10 mg/L anthracene in
70:30 acetonitrile/water
10 mL
WAT047685
Ammonium Formate Concentrate
5000 mM Ammonium Formate in a
Water w/ 3.8% Formic acid matrix.
This allows the end user to dilute the
concentrate 10 mL to 1 L before using to
reach a 50 mM concentration.
10 mL
186007081
Kit contains seven 10 mL vials of varying concentrations of anthracene in
80:20 acetonitrile/water:
(1) 0.5 pg/μL
(1) 1.0 pg/μL
(2) 5.0 pg/μL
(1) 10.0 pg/μL
(1) 2.5 ng/mL
(1) 2.5 μg/mL
One blank 10 mL vial of 80:20 acetonitrile/H
2
0