Care and use manual – Waters LCMS Quality Control Reference Materials User Manual
Page 2
II. STORAGE AND STABILIT Y
The standard mix comes in a Waters Crimp Top vial for direct injection
and contains 500 µL total in 1:10 in ACN:LC/MS Grade Water.
The standard mix is shipped at ambient temperatures. It is
highly recommended that upon receipt, the standard should be
refrigerated at 4 °C for short term and for long term stored frozen.
III. EXAMPLES OF USING T HE LCMS QC RM
In this section, two example methods are discussed to allow the
user to get an idea and understanding of how this standard can
be used under different conditions. The user is not aiming for an
exact replicate of these LC/MS chromatograms, however this is
provided as guidance. The advantage of this standard is that it can
be used with any column under different LC conditions. Therefore,
based on the information shown below, the user can customize this
standard to their specific method.
Shown in Figure 2 is a representative MS Spectrum under the
following conditions listed in Table 2.:
Table 2: Gradient Table for Figure 1
Time/min
Flow rate/
mL min
%A
%B
Curve
0.0
0.5
100
0
–
3.5
0.5
0
100
6
4.5
0.5
100
0
11
Total analysis time = 5 min
Column: ACQUITY
®
HSS T3, 2.1 x 100 mm, 1.8 µm
Mobile phase A:
Water + 0.1 % formic acid
Mobile phase B:
Acetonitrile
Injection volume: 1 µL
Acetaminophen
Sulfaguanidine
Sulfadimethoxine
Val -Tyr -Val
Verapamil
Terfenadine
Leucine -Enkephalin
Reserpine
Caffeine
Acetaminophen
Caffeine
Sulfaguanidine
Sulfadimethoxine
Val -Tyr -Val
Verapamil
Terfenadine
Leucine-Enkephalin
Reserpine
Table 3: Gradient Table for Figure 2
Time/min
Flow rate/
mL min
%A
%B
Curve
0.0
0.5
100
0
–
3.5
0.5
0
100
6
4.5
0.5
100
0
11
Total analysis time = 5 min
Figure 1: TOF MS ES+ Spectrum of the LCMS QCRM Standard using ACN Mobile Phase
with a SYNAPT
®
G2-S MS operated in positive ion mode in high resolution mode
Figure 2: TOF MS ES+ Spectrum of the LCMS QCRM Standard using Methanol
Mobile Phase with a SYNAPT G2-S MS operated in positive ion mode in high
resolution mode
Column:
ACQUITY HSS T3, 2.1 x 100 mm, 1.8 µm
Mobile phase A: Water + 0.1 % Formic acid
Mobile phase B:
Methanol + 0.1 % Formic acid
Injection volume: 1 µL
2
[ CARE AND USE MANUAL ]
LCMS QC Reference Standard