Waters MetID Small Molecule Standard Mix - Manual (Page 2)

MetID Standard Mix 2
[ CARE AND USE MANUAL ]
moderate precautions are taken and the samples, once reconstituted,
are stored properly at -10°C (and analyzed within an autosampler
set to 10°C or lower) the standard is typically stable for at least one
week. Beyond this time for performance testing and evaluation, fresh
re-preparation of the standard is recommended.
III. Reconstitution of the standard solution for testing
1. To the vial of Lyophilized Waters MetID Standard Mix, add 1 mL
of Methanol.
2. Recap and then agitate the vial contents (e.g. via shaking or
ultrasonic bath), to ensure the lyophilized powder dissolves in
the methanol.
3. This standard can be further diluted as per the UNIFI
Metabolite Identification Test Protocol, which documents
formal standard curve, PDA and UNIFI system performance
testing.
1
IV. Example of Using Omeprazole Standard Mix
Shown in figure 2 is a representative chromatogram under standard
chromatography conditions.
V. ORDERING INFORMATION
To order these products, contact your nearest subsidiary, or visit
www. waters.com and click on Order Center.
Description Part number
MetID Standard Mix 186006550
Formic Acid 186006691
VI. References
1 Metabolite Identification Application Solution Quick Start P/N 715003759.
2 Maton, P.N., Omeprazole Review Article, N. Engl J Med 1991; 324:965-975.
Figure 2. Omeprazole Standard Mix (33.3 ng/mL) extracted
ion chromatogram acquired on an ACQUITY UPLC I-Class
coupled to a Xevo
® G2-S QTof Mass Spectrometer System
and processed using UNIFI Scientific Information System.