Waters MassPREP Peptide Mix Standard - Manual (Page 2)

Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
[ CARE AND USE MANUAL ]
Waters, The Science of What’s Possible, MassPREP, MassLynx, and BioSuite are registered trademarks of Waters Corporation.
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©2014 Waters Corporation.Produced in the U.S.A. August 2014 715001703EN TC-PDF
II. STORAGE AND STABILIT Y
The lyophilized powder is stable at room temperature until
the expiration date printed on the label. The sample can be
reconstituted in 0.1% aqueous formic acid (for electrospray or
LC/MS applications) or in 0.1% aqueous trifluoroacetic acid
(for MALDI and LC/UV applications), the solution is stable
for one week when stored at 8 °C. Stability can be extended
by freezing samples at -20 °C. Note: The contamination of
sample with endo- and exopeptidase enzymes may cause
rapid sample degradation.
III. RECONSTITUTION OF MASSP REP
PEPTIDE MIX TURE
The peptide mixture contains approximately 1.5 µg (~1 nmole)
of each peptide. When reconstituted in 100 µL of solvent (0.1%
aqueous trifluoroacetic acid, or 0.1% aqueous formic acid),
the final sample concentration is ~15 µg/ml per peptide.
The suggested sample injection volumes for LC columns with
different internal diameters are summarized in Table 2.
Reconstitution
Volume (µl)
Column I.D.
(mm)
Injection
Volume
(µl)
Injected Mass
Per Peptide (ng)
Avg. Injected
Mass Per
Peptide
(pmole) *
100 4.6 75.0 1125.0 750.0
100 2.1 15.6 234.5 156.3
100 1.0 3.5 53.2 35.4
100 0.32 0.4 5.4 3.6
100 0.18 0.1 1.7 1.1
100 0.075 0.02 0.3 0.2
Table 2: Recommended Mass Load for LC Columns Evaluation.
IV. MSDS INFORMATION
Waters Peptide Mixture contains various non hazardous peptides
in powder form. They are for laboratory use only; solely for
purposes of scientific experimentation, analysis, research
or development. Waters Peptide Mixture should be used only
as directed and in accordance with good laboratory practice.
All Waters Peptide Mixtures are non hazardous as defined by
OSHA’s Hazard Communication Standard, 29 CFR 1910.1200(c)
and Appendix A and B, and are thus exempt from the requirements
of the Hazard Communication Standard, including the requirement
to supply MSDSs.
V. ORDERING INFORMATION
Item Part Number Quantity
Peptide Mixture 186002337 1
Peptide Mixture (5-pack) 186002338 5