Waters AccQ-Tag Ultra C18 Column - Manual (Page 2)

[ CARE AND USE MANUAL ]
2 AccQ•Tag Ultra C
18
Column
II. GET TING START ED AND COLUMN USE
Please refer to the Waters UPLC Amino Acid Analysis Solution
System Guide (P/N 71500129702) for detailed information. This
can be found by searching the literature code on www.waters.com.
III. COLUMN CLEANING AND STORAGE
a. Cleaning
When required, the AccQ•Tag Ultra C
18
column can be safely
cleaned with 100% acetonitrile at 0.5 mL/min for 30 minutes.
b. Storage
Please refer to the Waters UPLC Amino Acid Analysis Solution
System Guide (P/N 71500129702) for details on short vs longer
term column storage conditions.
IV. INT RODUCING ECORD INT ELLIGENT
CHIP T ECHNOLOGY
a. Introduction
The eCord™ intelligent chip is a new technology that will provide
the history of a column’s performance throughout its lifetime. The
eCord will be permanently attached to the column to assure that
the column’s performance history is maintained in the event that
the column is moved from one instrument to another.

eCord Intelligent
Figure 1: Waters eCord Intelligent Chip
At the time of manufacture, tracking and quality control
information will be downloaded to the eCord. This includes the
conditions and results for the Performance Test Chromatogram.
Storing this information on the chip will eliminate the need for a
paper Certificate of Analysis. Once the user installs the column,
the software will automatically download key parameters into a
column history file stored on the chip. In this manual, we explain
how the eCord will provide a solution for easily tracking the history
of the columns, reduce the frustration of paperwork trails, and
give customers the reassurance that a well performing column is
installed onto their instruments.
b. Installation
Install the column into the column heater. Plug the eCord into
the side of the column heater. Once the eCord is inserted into the
column heater, the identification and overall column usage infor-
mation will be available in the ACQUITY Console, allowing
the user to access column information on their desktop.
eCord Inserted into Side of Column Heater

Figure 2: Determination of Gradient Delay Volume