Calibration slowness and failure – Luminex MAGPIX Installation and Hardware User (IVD) User Manual
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Troubleshooting Procedures
Calibration Slowness and Failure
Calibration problems can result from a variety of causes, many of them easily correctable
human errors.
TABLE 6.
Possible Cause
Corrective Action
Possible Calibration Microsphere Causes:
The calibration microspheres are not fully
resuspended.
Vortex the calibration vials to resuspend
the microspheres.
The wrong lot number or target values
were entered in the software.
Correct the lot number and target values
in the software.
The calibration microspheres are in the
wrong well.
Change the well setting in the software.
There are not enough calibration
microspheres in the well.
Add at least five drops of calibration
microspheres to the well. For accurate
drop volume, hold the vial upside down at
a 90 degree angle to the microtiter plate
while dispensing them.
You are using the incorrect calibration
microspheres.
Use the xMAP MAGPIX Calibration Kit.
The calibration microsphere lot is expired.
Substitute an unexpired bottle of calibrator
microspheres.
Possible Sample Probe Causes:
The sample probe height is incorrect.
Perform an automatic sample probe
height adjustment.
The sample probe is clogged.
The probe fitting is loose.
Tighten the probe fitting.
Other Possible Causes:
There is a partial clog in the instrument.
There is air in the instrument.
Perform an automatic sample probe
height adjustment. Use the software to run
a prime command three times, an alcohol
flush command twice, then a wash
command three times with distilled water.
Make sure the Drive Fluid coil is not
pinched.
The sample valve is faulty.
Contact Technical Support.
There is a problem internal to the
instrument.
Review the log of calibration reports.
Check for dramatic changes in
temperature or voltage. If any of these are
present, contact Technical Support.
No events are being collected during
calibration.
Check the drive and waste fluid levels.
Verify that tubing for both containers is
tightly connected.
Check for sample probe problems.