Electronics, Optics, Fluidics – Luminex MAGPIX Installation and Hardware User (IVD) User Manual
Page 58: Microtiter plates, Microspheres
Luminex IVD MAGPIX Hardware Installation and User Manual
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Operating altitude: up to 2400m (7874 ft) above mean sea level
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Shipping temperature: 0-50°C (32-122°F)
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Storage temperature: 10-40°C (50-104°F)
Electronics
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USB 2.0-compatible communications link for fast data transfer
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Input voltage range: either 100-120 V~, 2.0 A 50/60 Hz or 200-240 V~, 1.0 A 50/60 Hz
Optics
•
Reporter channel detection: A/D resolution 16 bits
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Reporter detector: CCD, detection bandwidth of 566 to 614 nm
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Classification detector: CCD
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Limit of Detection (LOD): For the reporter channel, using a blank microsphere from
region 078,
700 molecules of phycoerythrin (PE) per microsphere
•
Dynamic Range: For the reporter channel, using a microsphere from region 078,
3.0
decades
•
Efficiency: Classification Channels:
80%
Fluidics
•
Sample load rate: 20
L to 500 L per second
•
Sample uptake volume: 20 to 200
L
•
Well-to-well carryover:
<
4%
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Sample uptake accuracy: +/- 5%
Microtiter Plates
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Microtiter plate must be 96-well, not to exceed 1” (2.54cm) in height, including heater
block.
•
Microtiter plate must be compatible with the microtiter heater block temperature when
the heater block is in use.
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All microtiter plates have standard width (85.5mm) and length (127.9 mm). Depth
varies depending on the type of well. Maximum allowable depth is 1” (2.54cm). Plates
must have minimum 0.06” (1.5mm) lip height, standard distance from well center to
well center (9mm) and standard distance from A1 center to plate center in both length
and width. To be compatible in size with the microtiter heater block, the plate must fit
into the heater block so that the top is flush with the heater block.
Microspheres
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Distinguishes 1 to 50 unique MagPlex microspheres in a single sample
•
Misclassification Rate
2%
•
Classification Rate
80%
•
Detects and distinguishes surface reporter fluorescence emissions at 590 nm +/- 24
nm on the surface of 1 to 50 unique MagPlex microsphere in a single sample
•
Soluble background fluorescence emission at 590 nm +/- 24 nm automatically
subtracted from fluorescence intensity values