Multichannel Systems Cardio2D Manual User Manual

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Cardio2D Manual

Second section: Click one of the three color coded "Select Stimulus" icons

to select

the respective stimulus pattern for further processing. You can define the location of the selected
stimulus by clicking on electrodes in the electrode panel or by drawing rectangles over the desired
area. Each stimulation electrode accepts one stimulus pattern at a time. It is not possible to
overlap patterns, but to overwrite them with a new selection or to clear the sites. To remove the

currently selected stimulus from all sites, please use the "Clear selected stimulus sites" icon

All created sites of the selected pattern will be removed. To remove stimuli from single electrodes
only, cancel the current stimulus pattern selection first by clicking on the “Select Stimulus” icon
again, then click on the electrode you would like to remove.

Clicking the “Define Stimulus” icon

will open a new dialog enabling the definition of the

stimulus pattern as explained on the following pages. To start stimulation with the currently

selected pattern only, click the “Start” icon

in the second section of the tool bar.

Third section: To clear all sites, click the "Clear all stimulus sites" icon

Start all stimulus patterns simultaneously via the "Start" icon

in this section.

Stimulation of electrodes produces stimulation artefacts, which can be eliminated. To select the

blanking artefact suppression, please click the "Blanking" icon

. ”Blanking” will blank all

recording electrodes on a MEA while a stimulus pattern is applied to any electrode of the same

MEA. Choosing the ”Dedicated Electrode” icon

all selected electrodes will be defined to

be dedicated stimulation electrodes. These electrodes will be permanently isolated from the
amplifier, hence recording from these electrodes will be stopped.

Note: It is not possible to select electrode 15 for stimulation, because this electrode is connected
to the internal reference.

Viability Test

Before you start recording, a viability test is recommended. The data is not recorded. During
viability test it is possible to change the stimulation electrodes and stimulation pattern without
stopping the display of the data.

Such changes are not possible during data acquisition! You first have to set stimulation electrodes
and stimulation parameters which are fix and then start the experiment.

Important: If you remove electrodes from the screen, data streams of these electrodes are not
saved in the data file.