Multichannel Systems Cardio2D Manual - Manual (Page 22)

Cardio2D Manual
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Control Window
The experimental protocol is visualized by three icons on the left orange control view.
The icons can be clicked for editing the protocol. Below the control view the log file
documents the ongoing experimental proceedings.
Summary Window
The "Summary" window is only available, if more than one MEA is connected. It presents
an overview about the layout of the MEAs which are connected and you can select or deselect
the recording of connected MEAs. The summary corresponds with the MEA tabbed pages in the
"MEA Window".
MEA Window
The "MEA Window" is divided into four single views. All of them display heartbeats in different
characteristics. If more than one MEA is connected to the experimental setup, each MEA can be
operated separately via the "MEA Tabbed Pages". Depending on the hardware up to nine MEAs
(for nine wells of the 256-9wellMEA) are available. Select the respective MEA in the "Summary"
window to display the data traces in the MEA window. If a MEA is disabled via check box in the
"Summary" window, the corresponding MEA tabbed page will be removed from the display,
and no data is recorded.
Five different types of MEAs are implemented: The standard MEA with an 8 x 8 electrode layout
grid (the diameter of the MEA electrodes and the spacing between them differs), a MEA with a
6x10 electrode layout grid, the 256MEA with a 16 x 16 layout grid, the 256-9wellMEA with nine
separate wells (shown in the picture above), or the EcoFlexMEA36 with 6 x 6 electrode layout grid.
The design of the MEA window is automatically adjusted to the type of the MEA, please see
chapter "Data Acquisition Settings" in First Use of Cardio2D after Installation.
Recorded data traces are displayed in the MEA electrodes pane. You can zoom to any channel
by clicking on a channel in the MEA electrodes plate. The zoomed channel is displayed in the
larger single channel view. The single channel view has three tabs, "FP" field potential, "HB"
heartbeat and "Ave" average. The heartbeat window shows either a diagram of the RR interval
or of the frequency of the heartbeats, the heart rate. The current values of the RRI or the
frequency are displayed above in red numbers.
The color map displays the propagation of a heartbeat from the pacemaker to the outer regions
of the cardiac cell culture or tissue. The isochrones are indicated by black lines. The colors indicate
the latency distribution over the MEA. Red color represents the pacemaker when using the color
map "jet inverse".
The latency / velocity window shows the latency of the heartbeat in units of μs or the velocity
in units of mm per ms. Both parameters are measured between the first and the last detected
electrode in the range. The current absolute values are displayed in red letters above the plot.